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Literature summary extracted from
- Rabbit liver glutathione reductase. Purification and properties (1979), Arch. Biochem. Biophys., 198, 241-246.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.8.1.7 | repeated freezing and thawing, stable | Oryctolagus cuniculus |
| 1.8.1.7 | urea, quite stable to | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.7 | 0.008 | - |
NADPH | - |
Oryctolagus cuniculus | |
| 1.8.1.7 | 0.058 | - |
GSSG | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 56000 | - |
SDS-PAGE, gel filtration, monomer, enzyme can also be active as dimer | Oryctolagus cuniculus |
| 1.8.1.7 | 108000 | - |
ultracentrifugation in glycerol density gradient, dimer, enzyme can also be active also as monomer | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 180 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.8.1.7 | -20°C, with several freeze-thawing cycles, more than 2 years, stable | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | - |
Oryctolagus cuniculus | glutathione + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | dimer | 2 * 56000, SDS-PAGE, enzyme can also be active as monomer | Oryctolagus cuniculus |
| 1.8.1.7 | monomer | 1 * 56000, SDS-PAGE, enzyme can also be active as dimer | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 75 | - |
1 h, 25% loss of activity | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 6.9 | - |
- |
Oryctolagus cuniculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 5.2 | 9 | at pH 5.2 and 9.0 about 20% of activity maximum | Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | FAD enzyme | Oryctolagus cuniculus | |
| 1.8.1.7 | NADPH | absolute specificity for NADPH | Oryctolagus cuniculus | |
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Release 2023.1 (January 2023)
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