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Literature summary extracted from

  • Bielawski, W.; Joy, K.W.
    Properties of glutathione reductase from chloroplasts and roots of pea (1986), Phytochemistry, 25, 2261-2265.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 Cu2+ root enzyme inhibited, chloroplast enzyme only slightly Pisum sativum
1.8.1.7 Fe2+ root enzyme inhibited, chloroplast enzyme only slightly Pisum sativum
1.8.1.7 N-ethylmaleimide root enzyme is slightly more sensitive than chloroplast enzyme Pisum sativum
1.8.1.7 p-hydroxymercuribenzoate chloroplast enzyme is slightly more sensitive than root enzyme Pisum sativum
1.8.1.7 Zn2+ root enzyme inhibited, chloroplast enzyme very slightly Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.0023
-
 NADPH root enzyme Pisum sativum
1.8.1.7 0.003
-
 NADPH chloroplast enzyme Pisum sativum
1.8.1.7 0.01
-
 GSSG root enzyme Pisum sativum
1.8.1.7 0.028
-
 GSSG chloroplast enzyme Pisum sativum
1.8.1.7 0.22
-
 NADP+ root and chloroplast enzyme Pisum sativum
1.8.1.7 7.7
-
 glutathione chloroplast enzyme Pisum sativum
1.8.1.7 8.2
-
 glutathione root enzyme Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.7 chloroplast
-
 Pisum sativum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 156000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Pisum sativum
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 leaf
-
 Pisum sativum
-
1.8.1.7 root
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 120
-
 purified enzyme Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH
-
 Pisum sativum glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 tetramer
-
 Pisum sativum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 additional information
-
 root enzyme more stable than chloroplast enzyme, oxidized form more stable than reduced form Pisum sativum
1.8.1.7 30
-
 reduced form of the enzyme highly unstable Pisum sativum
1.8.1.7 60 65 oxidized form stable Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7.7
-
 root enzyme Pisum sativum
1.8.1.7 8.2
-
 chloroplast enzyme Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.7 6.1 9.1 50% of maximal activity at pH 6.1 and 9.1, root enzyme Pisum sativum
1.8.1.7 6.9 9.2 pH 6.9: about 50% of activity maximum, pH 9.2: about 83% of activity maximum Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADH 20times higher activity with NADH than with NADPH Pisum sativum
1.8.1.7 NADPH 5% of the activity with NADH, 3fold higher affinity of root enzyme compared to chloroplast enzyme Pisum sativum