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Literature summary extracted from

  • Zirngibl, C.; Hedderich, R.; Thauer, R.K.
    N5,N10-Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity (1990), FEBS Lett., 261, 112-116.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.98.2 H2 inhibits formation of N5,N10-methenyltetrahydromethanopterin Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.2 0.02
-
 N5,N10-methylenetetrahydromethanopterin
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.2 Iron contains approximately 1 mol of iron per mol of enzyme Methanothermobacter thermautotrophicus
1.12.98.2 Nickel contains less than 0.1 mol Ni per mol of enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.2 43000
-
 x * 43000, SDS-PAGE Methanothermobacter thermautotrophicus
1.12.98.2 45000
-
 gradient PAGE Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.2 Methanothermobacter thermautotrophicus
-
-
-
1.12.98.2 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.2
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.2 750
-
-
 Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
1.12.98.2 4°C, pH 7.6, 50 mM Tris-HCl, 20% glycerol, under N2 gas phase, 50% loss of activity after 1 week Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ r Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ no reaction with: coenzyme F420, NAD+, NADP+ Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ no reaction with viologen dyes Methanothermobacter thermautotrophicus N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ r Methanothermobacter thermautotrophicus Marburg / DSM 2133 N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ no reaction with: coenzyme F420, NAD+, NADP+ Methanothermobacter thermautotrophicus Marburg / DSM 2133 N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ no reaction with viologen dyes Methanothermobacter thermautotrophicus Marburg / DSM 2133 N5,N10-methenyltetrahydromethanopterin + H2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.2 monomer x * 43000, SDS-PAGE Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.2 6.5
-
-
 Methanothermobacter thermautotrophicus