Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tano, T.; Sakai, K.; Sugio, T.; Imai, K.
    Purification and some properties of a ferrocytochrome c: hydrogen-peroxide oxidoreductase from Thiobacillus thiooxidans (1977), Agric. Biol. Chem., 41, 323-330.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.5 Ag+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans
1.11.1.5 azide
-
 Acidithiobacillus thiooxidans
1.11.1.5 CO 40% inhibition Acidithiobacillus thiooxidans
1.11.1.5 Cu2+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans
1.11.1.5 cyanide
-
 Acidithiobacillus thiooxidans
1.11.1.5 Hg2+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans
1.11.1.5 Pb2+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.5 Iron heme prosthetic group Acidithiobacillus thiooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.5 32000
-
 gel filtration Acidithiobacillus thiooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.5 Acidithiobacillus thiooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.5
-
 Acidithiobacillus thiooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.5 18.16
-
-
 Acidithiobacillus thiooxidans

Storage Stability

EC Number Storage Stability Organism
1.11.1.5 -20°C, 0.1 M phosphate buffer, pH 7.0, two months Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.5 ferrocytochrome c + H2O2 horse heart Acidithiobacillus thiooxidans ferricytochrome c + H2O
-
 ?
1.11.1.5 ferrocytochrome c + menadione menadione can be substituted by 1,4-naphthoquinone Acidithiobacillus thiooxidans ferricytochrome + oxidized menadione
-
 ?
1.11.1.5 NADH + H2O2
-
 Acidithiobacillus thiooxidans NAD+ + H2O
-
 ?
1.11.1.5 NADPH + H2O2
-
 Acidithiobacillus thiooxidans NADP+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.5 70
-
 15 min, 80% loss of peroxidizing activity, 50% loss of NADH oxidizing activity Acidithiobacillus thiooxidans
1.11.1.5 90
-
 inactivation Acidithiobacillus thiooxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.5 2 10
-
 Acidithiobacillus thiooxidans