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Literature summary extracted from

  • Karhunen, E.; Kantelinen, A.; Niku-Paavola, M.L.
    Mn-dependent peroxidase from the lignin-degrading white rot fungus Phlebia radiata (1990), Arch. Biochem. Biophys., 279, 25-31.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.13 H2O2 H2O2-dependent Phlebia radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.13 catalase inhibits oxidation of vanillylacetone completely, oxidation of NADH at high concentrations partially Phlebia radiata
1.11.1.13 diphosphate inhibits phenol red oxidation to 50%, NADH oxidation at high concentrations partially Phlebia radiata
1.11.1.13 additional information not inhibited by sodium diphosphate, mannitol, Mn3+-chelators Phlebia radiata
1.11.1.13 Superoxide dismutase inhibits phenol red oxidation to 50%, vanillylacetone oxidation to 90%, NADH oxidation at high concentrations partially Phlebia radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.13 extracellular
-
Phlebia radiata
-
-
1.11.1.14 extracellular or losely associated to cell membrane Phlebia radiata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.14 Iron
-
Phlebia radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 49000
-
x * 49000, SDS-PAGE Phlebia radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 Phlebia radiata
-
?
-
?
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 Phlebia radiata 79 / ATCC 64658
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phlebia radiata
-
white rot fungus, strain 79
-
1.11.1.14 Phlebia radiata
-
-
-
1.11.1.14 Phlebia radiata 79 / ATCC 64658
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein 10% w/w carbohydrates Phlebia radiata

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13
-
Phlebia radiata

Reaction

EC Number Reaction Comment Organism Reaction ID
1.11.1.13 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O mechanism Phlebia radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.14 culture supernatant
-
Phlebia radiata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 Mn2+ + H+ + H2O2 completion of MnP catalytic cycle requires Mn2+ Phlebia radiata Mn3+ + H2O Mn3+ oxidizes vanillylacetone ?
1.11.1.13 Mn2+ + H+ + H2O2 completion of MnP catalytic cycle requires Mn2+ Phlebia radiata Mn3+ + H2O Mn3+ oxidizes phenol red ?
1.11.1.13 Mn2+ + H+ + H2O2 completion of MnP catalytic cycle requires Mn2+ Phlebia radiata Mn3+ + H2O Mn3+ oxidizes a variety of phenols ?
1.11.1.13 Mn2+ + H+ + H2O2 completion of MnP catalytic cycle requires Mn2+ Phlebia radiata Mn3+ + H2O Mn3+ oxidizes several methoxylated and hydroxylated phenolic compounds ?
1.11.1.13 additional information poor substrates: benzoate, benzaldehyde or benzyl alcohol Phlebia radiata ?
-
?
1.11.1.13 additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phlebia radiata ?
-
?
1.11.1.13 additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates Phlebia radiata ?
-
?
1.11.1.13 additional information catalytic cycle with oxidized intermediates MnP compound I and II Phlebia radiata ?
-
?
1.11.1.13 additional information no activity with veratryl alcohol Phlebia radiata ?
-
?
1.11.1.13 additional information Mn-dependent oxidation of phenols requires superoxide anion and H2O2, phenolic hydroxyl group is essential Phlebia radiata ?
-
?
1.11.1.13 additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ Phlebia radiata ?
-
?
1.11.1.13 additional information in presence of Mn2+ enzyme oxidizes various organic compounds Phlebia radiata ?
-
?
1.11.1.13 additional information poor substrates: benzoate, benzaldehyde or benzyl alcohol Phlebia radiata 79 / ATCC 64658 ?
-
?
1.11.1.13 additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phlebia radiata 79 / ATCC 64658 ?
-
?
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2
-
Phlebia radiata ?
-
?
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2
-
Phlebia radiata 79 / ATCC 64658 ?
-
?
1.11.1.14 3,4-dimethoxybenzyl alcohol + H2O2 veratryl alcohol Phlebia radiata 3,4-dimethoxybenzaldehyde + H2O
-
?
1.11.1.14 3,4-dimethoxybenzyl alcohol + H2O2 veratryl alcohol Phlebia radiata 79 / ATCC 64658 3,4-dimethoxybenzaldehyde + H2O
-
?
1.11.1.14 additional information oxidation of various phenolic and non-phenolic lignin model-compounds Phlebia radiata ?
-
?
1.11.1.14 additional information oxidation of various phenolic and non-phenolic lignin model-compounds Phlebia radiata 79 / ATCC 64658 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.13 ? x * 49000, SDS-PAGE Phlebia radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.13 additional information
-
assay at room temperature Phlebia radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 additional information
-
pI: 3.8 Phlebia radiata
1.11.1.13 4
-
oxidation of NADH Phlebia radiata
1.11.1.14 additional information
-
pI: 3.2 (isozyme LI), 3.8 (LII), 3.9 (LIII) Phlebia radiata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.13 3 6 stable Phlebia radiata

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.13 heme iron protoporphyrin IX prosthetic group Phlebia radiata