EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | H2O2 | H2O2-dependent | Phlebia radiata |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | catalase | inhibits oxidation of vanillylacetone completely, oxidation of NADH at high concentrations partially | Phlebia radiata | |
1.11.1.13 | diphosphate | inhibits phenol red oxidation to 50%, NADH oxidation at high concentrations partially | Phlebia radiata | |
1.11.1.13 | additional information | not inhibited by sodium diphosphate, mannitol, Mn3+-chelators | Phlebia radiata | |
1.11.1.13 | Superoxide dismutase | inhibits phenol red oxidation to 50%, vanillylacetone oxidation to 90%, NADH oxidation at high concentrations partially | Phlebia radiata |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.13 | extracellular | - |
Phlebia radiata | - |
- |
1.11.1.14 | extracellular | or losely associated to cell membrane | Phlebia radiata | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.14 | Iron | - |
Phlebia radiata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 49000 | - |
x * 49000, SDS-PAGE | Phlebia radiata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | Phlebia radiata | - |
? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | Phlebia radiata 79 / ATCC 64658 | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.13 | Phlebia radiata | - |
white rot fungus, strain 79 | - |
1.11.1.14 | Phlebia radiata | - |
- |
- |
1.11.1.14 | Phlebia radiata 79 / ATCC 64658 | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.11.1.13 | glycoprotein | 10% w/w carbohydrates | Phlebia radiata |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.13 | - |
Phlebia radiata |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | mechanism | Phlebia radiata |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.11.1.14 | culture supernatant | - |
Phlebia radiata | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes phenol red | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phlebia radiata | Mn3+ + H2O | Mn3+ oxidizes several methoxylated and hydroxylated phenolic compounds | ? | |
1.11.1.13 | additional information | poor substrates: benzoate, benzaldehyde or benzyl alcohol | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | catalytic cycle with oxidized intermediates MnP compound I and II | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | no activity with veratryl alcohol | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | Mn-dependent oxidation of phenols requires superoxide anion and H2O2, phenolic hydroxyl group is essential | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phlebia radiata | ? | - |
? | |
1.11.1.13 | additional information | poor substrates: benzoate, benzaldehyde or benzyl alcohol | Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
1.11.1.13 | additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | - |
Phlebia radiata | ? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | - |
Phlebia radiata 79 / ATCC 64658 | ? | - |
? | |
1.11.1.14 | 3,4-dimethoxybenzyl alcohol + H2O2 | veratryl alcohol | Phlebia radiata | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
1.11.1.14 | 3,4-dimethoxybenzyl alcohol + H2O2 | veratryl alcohol | Phlebia radiata 79 / ATCC 64658 | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phlebia radiata | ? | - |
? | |
1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phlebia radiata 79 / ATCC 64658 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.13 | ? | x * 49000, SDS-PAGE | Phlebia radiata |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
assay at room temperature | Phlebia radiata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | additional information | - |
pI: 3.8 | Phlebia radiata |
1.11.1.13 | 4 | - |
oxidation of NADH | Phlebia radiata |
1.11.1.14 | additional information | - |
pI: 3.2 (isozyme LI), 3.8 (LII), 3.9 (LIII) | Phlebia radiata |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 3 | 6 | stable | Phlebia radiata |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | heme | iron protoporphyrin IX prosthetic group | Phlebia radiata |