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Literature summary extracted from
- Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase (1984), Proc. Natl. Acad. Sci. USA, 81, 2280-2284.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.11.1.14 | lyophilization, stable to | Phanerodontia chrysosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.14 | H2O2 | above 5 mM | Phanerodontia chrysosporium |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.14 | 0.03 | - |
H2O2 | - |
Phanerodontia chrysosporium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.11.1.14 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.14 | Iron | 1 mol protoheme IX per mol enzyme | Phanerodontia chrysosporium | |
| 1.11.1.14 | Iron | 1.08 atom Fe per enzyme molecule | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.14 | 42000 | - |
x * 42000, SDS-PAGE | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | Phanerodontia chrysosporium | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.14 | Phanerodontia chrysosporium | - |
strains BKM-F-1767 | - |
| 1.11.1.14 | Phanerodontia chrysosporium BKM-F-1767 | - |
strains BKM-F-1767 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.14 | - |
Phanerodontia chrysosporium |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.11.1.14 | culture supernatant | - |
Phanerodontia chrysosporium | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.14 | 8.4 | - |
veratryl alcohol | Phanerodontia chrysosporium |
| 1.11.1.14 | 11.4 | - |
1,2-bis(3,4-di-methoxyphenyl)propane-1,3-diol | Phanerodontia chrysosporium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.11.1.14 | -20°C, stable as lyophilized powder | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.14 | additional information | of the aryl-CalphaHOH-CbetaHR-CgammaH2OH-type (R being aryl or O-aryl) | Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.14 | additional information | intradiol cleavage in phenylglycol structures, hydroxylation of benzylic methylene groups, oxidative coupling of phenols, all reactions require H2O2, Calpha-Cbeta cleavage and methylene hydroxylation involve substrate oxygenation, the oxygen atom originates from O2 not H2O2: thus the enzyme acts as oxygenase which requires H2O2 | Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.14 | additional information | with concomitant insertion of 1 atom of molecular oxygen | Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.14 | additional information | oxidation of benzyl alcohols to aldehydes or ketones | Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
| 1.11.1.14 | additional information | of the aryl-CalphaHOH-CbetaHR-CgammaH2OH-type (R being aryl or O-aryl) | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
| 1.11.1.14 | additional information | intradiol cleavage in phenylglycol structures, hydroxylation of benzylic methylene groups, oxidative coupling of phenols, all reactions require H2O2, Calpha-Cbeta cleavage and methylene hydroxylation involve substrate oxygenation, the oxygen atom originates from O2 not H2O2: thus the enzyme acts as oxygenase which requires H2O2 | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
| 1.11.1.14 | additional information | with concomitant insertion of 1 atom of molecular oxygen | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
| 1.11.1.14 | additional information | oxidation of benzyl alcohols to aldehydes or ketones | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.14 | ? | x * 42000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.14 | 37 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.14 | additional information | - |
pI: 3.5 | Phanerodontia chrysosporium |
| 1.11.1.14 | 3 | - |
- |
Phanerodontia chrysosporium |
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