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Literature summary extracted from

  • Coyle, C.L.; Zumft, W.G.; Kroneck, P.M.H.; Körner, H.; Jakob, W.
    Nitrous oxide reductase from denitrifying Pseudomonas perfectomarina. Purification and properties of a novel multicopper enzyme (1985), Eur. J. Biochem., 153, 459-467.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 O2 unpurified enzyme is rapid inactivated at air Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu 8 atoms per mol enzyme Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.4 74000
-
 2 * 74000, SDS-PAGE Pseudomonas stutzeri
1.7.2.4 118000
-
 gel filtration Pseudomonas stutzeri
1.7.2.4 130000
-
 non denaturating PAGE Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Pseudomonas stutzeri
-
 nitrogen + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Pseudomonas stutzeri
-
 ATCC 14405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4 a pink form is isolated when the purification is done aerobically, a purple form when the purification is under anaerobic conditions Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor
-
 Pseudomonas stutzeri nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor photochemically-reduced benzyl viologen as electron donor Pseudomonas stutzeri nitrogen + H2O + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 homodimer 2 * 74000, SDS-PAGE Pseudomonas stutzeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.4 25
-
 85% inactivation after 20 h Pseudomonas stutzeri