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Literature summary extracted from

  • Hole, U.H.; Vollack, K.U.; Zumft, W.G.; Eisenmann, E.; Siddiqui, R.A.; Friedrich, B.; Kroneck, P.M.H.
    Characterization of the membranous denitrification enzymes nitrite reductase (cytochrome cd1) and copper-containing nitrous oxide reductase from Thiobacillus denitrificans (1996), Arch. Microbiol., 165, 55-61.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 Acetylene at 10 mM 80% inhibition Thiobacillus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.1 membrane equally distributed between the soluble- and the membrane fraction Thiobacillus denitrificans 16020
-
1.7.2.4 membrane
-
 Thiobacillus denitrificans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu
-
 Thiobacillus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 66000
-
 2 * 66000, SDS-PAGE Thiobacillus denitrificans
1.7.2.1 100000
-
 gel filtration Thiobacillus denitrificans
1.7.2.4 73000
-
 2 * 73000, SDS-PAGE Thiobacillus denitrificans
1.7.2.4 160000
-
 gel filtration Thiobacillus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Thiobacillus denitrificans
-
 nitrogen + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Thiobacillus denitrificans
-
-
-
1.7.2.4 Thiobacillus denitrificans
-
 DSM 807
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1
-
 Thiobacillus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 2.1
-
 nitrite reduction, elctron donor methyl viologen Thiobacillus denitrificans
1.7.2.4 0.05
-
-
 Thiobacillus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 NO2 + reduced methyl viologen
-
 Thiobacillus denitrificans NO + oxidized methylviologen
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor
-
 Thiobacillus denitrificans nitrogen + H2O + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.1 dimer 2 * 66000, SDS-PAGE Thiobacillus denitrificans
1.7.2.4 homodimer 2 * 73000, SDS-PAGE Thiobacillus denitrificans