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Literature summary extracted from

  • Sato, K.; Okubo, A.; Yamazaki, S.
    Characterization of a multi-copper enzyme, nitrous oxide reductase, from Rhodobacter sphaeroides f. sp. denitrificans (1998), J. Biochem., 124, 51-54.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 EDTA complete inhibition at 0.1 mM Cereibacter sphaeroides
1.7.2.4 Ni2+ 50% inhibition at 0.1 mM Cereibacter sphaeroides
1.7.2.4 Zn2+ complete inhibition at 0.1 mM Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu 7.0 atoms per dimer, needs 0.001 mM Cu2+ for full activity Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.4 66180
-
 2 * 66180, MALDI-TOF Cereibacter sphaeroides
1.7.2.4 67000
-
 2 * 67000, SDS-PAGE Cereibacter sphaeroides
1.7.2.4 105000
-
 gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Cereibacter sphaeroides
-
 nitrogen + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Cereibacter sphaeroides
-
 f. sp. denitrificans IL106
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4 purification aerobically at 4°C Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 44
-
 aerobic form Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor
-
 Cereibacter sphaeroides nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor photochemically-reduced benzyl viologen as electron donor Cereibacter sphaeroides nitrogen + H2O + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 homodimer 2 * 67000, SDS-PAGE Cereibacter sphaeroides
1.7.2.4 homodimer 2 * 66180, MALDI-TOF Cereibacter sphaeroides