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Literature summary extracted from

  • Sato, K.; Okubo, A.; Yamazaki, S.
    Anaerobic purification and characterization of nitrous oxide reductase from Rhodobacter sphaeroides f. sp. denitrificans IL106 (1999), J. Biochem., 125, 864-868.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.2.4 turnover dependent inactivation, promoted by zinc ions at 0.01 mM Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 O2 turnover dependent inactivation Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.4 66180
-
 2 * 66180, MALDI-TOF Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Cereibacter sphaeroides
-
 nitrogen + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Cereibacter sphaeroides
-
 f. sp. denitrificans IL106
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4 purification under anaerobic conditions Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 44
-
 aerobic form Cereibacter sphaeroides
1.7.2.4 78
-
 anaerobic form Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
1.7.2.4 4°C, retained most of its activity after aerobic storage for 2 months without any additives Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor
-
 Cereibacter sphaeroides nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor dithionite-reduced benzyl viologen as electron donor Cereibacter sphaeroides nitrogen + H2O + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 homodimer 2 * 66180, MALDI-TOF Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.4 60
-
-
 Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.2.4 70
-
 most of activity lost Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.4 8.5 9 with reduced benzyl viologen as electron donor Cereibacter sphaeroides