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Literature summary extracted from

  • Rasmussen, T.; Berks, B.C.; Sanders-Loehr, J.; Dooley, D.M.; Zumft, W.G.; Thomson, A.J.
    The catalytic center in nitrous oxide reductase, CuZ, is a copper-sulfide cluster (2000), Biochemistry, 39, 12753-12756.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.7.2.4 degradation plays a critical enviromental role in preventing release into the atmosphere of the potent greenhouse gas nitrous oxide Pseudomonas stutzeri
1.7.2.4 degradation plays a critical enviromental role in preventing release into the atmosphere of the potent greenhouse gas nitrous oxide Paracoccus pantotrophus

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.4 C622D no activity, distorted CuA-centre Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu wild-type 9.9 atoms per protein dimer Pseudomonas stutzeri
1.7.2.4 Cu wild-type 10.5 atoms per protein dimer Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Pseudomonas stutzeri
-
nitrogen + H2O + acceptor
-
?
1.7.2.4 nitrous oxide + reduced acceptor Paracoccus pantotrophus
-
nitrogen + H2O + acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Paracoccus pantotrophus
-
-
-
1.7.2.4 Pseudomonas stutzeri
-
wild-type, C622D-mutant laccking copper in the CuA-site and N2OR V a mutant which lacks CuZ
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 5.5
-
wild-type Pseudomonas stutzeri
1.7.2.4 6.9
-
-
Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor
-
Pseudomonas stutzeri nitrogen + H2O + acceptor
-
?
1.7.2.4 nitrous oxide + reduced acceptor
-
Paracoccus pantotrophus nitrogen + H2O + acceptor
-
?