Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rasmussen, T.; Berks, B.C.; Butt, J.N.; Thomson, A.J.
    Multiple forms of the catalytic centre, Cuz, in the enzyme nitrous oxide reductase from Paracoccus pantotrophus (2002), Biochem. J., 364, 807-815.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 O2 inactivation is faster in the crude extract than solutions of the purified enzyme, acceleration takes place if the enzyme is turning over Paracoccus pantotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu one dinuclear centre CuA and a copper cluster CuZ in which four copper ions are litigated by seven histidine imidazoles and a bridging inorganic sufide Paracoccus pantotrophus
1.7.2.4 Cu CuA can exist in two oxidation forms [Cu1.5+ - Cu1.5+] and [Cu1+ - Cu1+] Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Paracoccus pantotrophus
-
 nitrogen + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Paracoccus pantotrophus
-
 formerly Thiosphaera pantotropha
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 1.6
-
 anaerobic form pre-reduced with ascorbate Paracoccus pantotrophus
1.7.2.4 2.6
-
 anaerobic form pre-oxidized with ferricyanide Paracoccus pantotrophus
1.7.2.4 2.8
-
 anaerobic form pre-reduced with dithionite Paracoccus pantotrophus
1.7.2.4 8.5
-
 aerobic form pre-oxidized with ferricyanide Paracoccus pantotrophus
1.7.2.4 8.8
-
 aerobic form pre-reduced with dithionite Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor
-
 Paracoccus pantotrophus nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor methyl viologen as electron donor, activity after incubation with ferricyanide, ascorbate or dithionite Paracoccus pantotrophus nitrogen + H2O + acceptor
-
 ?