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Literature summary extracted from

  • Saari, L.L.; Klucas, R.V.
    Ferric leghemoglobin reductase from soybean root nodules (1984), Arch. Biochem. Biophys., 231, 102-113.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.6 acetate slight Glycine max
1.6.2.6 iodoacetamide
-
 Glycine max
1.6.2.6 nicotinate
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.6 0.0095
-
 ferrileghemoglobin
-
 Glycine max
1.6.2.6 0.0188
-
 NADH
-
 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.6 cytosol
-
 Glycine max 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.6 54000
-
 2 * 54000, SDS-PAGE Glycine max
1.6.2.6 83000
-
 equilibrium ultracentrifugation Glycine max
1.6.2.6 100000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.6 NAD(P)H + ferrileghemoglobin Glycine max
-
 NAD(P)+ + ferroleghemoglobin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.6 Glycine max
-
 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.6
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.6 root nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.6 0.218
-
 reduction of ferrileghemoglobin Glycine max

Storage Stability

EC Number Storage Stability Organism
1.6.2.6 0°C, 37 days, 96% activity retains Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.6 2 ferricytochrome c + NAD(P)H reduced at 53% of leghemoglobin rate Glycine max 2 ferrocytochrome c + NAD(P)+ + H+
-
 ?
1.6.2.6 NAD(P)H + ferrileghemoglobin
-
 Glycine max NAD(P)+ + ferroleghemoglobin
-
 ?
1.6.2.6 NAD(P)H + ferrileghemoglobin 31% activity with NADPH Glycine max NAD(P)+ + ferroleghemoglobin
-
 ?
1.6.2.6 NAD(P)H + ferrimyoglobin
-
 Glycine max NAD(P)+ + ferromyoglobin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.6 dimer 2 * 54000, SDS-PAGE Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.6 additional information
-
 heat labile Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.6 5.2
-
 decrease of activity from 100% at pH 5.2, to 30% at pH 6.7, no measurements below 5.2 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.6 NADH
-
 Glycine max
1.6.2.6 NADPH 31% of NADH activity Glycine max