BRENDA - Enzyme Database show

A pyridine nucleotide-hydroxylamine reductase from Neurospora

Zucker, M.; Nason, A.; J. Biol. Chem. 213, 463-478 (1955)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.7.1.10
dialysis against 3 mM sodium diphosphate, 1 mM cysteine solution, pH 8, 2 h, less than 50% loss of activity, against 10 mM phosphate, pH 8, 1 h, complete loss of activity
Neurospora crassa
1.7.1.10
little or no activity in phosphate buffer unless 1 mM EDTA is included in the reaction mixture
Neurospora crassa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.10
2,2'-dipyridyl
-
Neurospora crassa
1.7.1.10
8-hydroxyquinoline
-
Neurospora crassa
1.7.1.10
Cu2+
-
Neurospora crassa
1.7.1.10
KCN
0.1 mM, complete inhibition
Neurospora crassa
1.7.1.10
NaN3
-
Neurospora crassa
1.7.1.10
o-phenanthroline
-
Neurospora crassa
1.7.1.10
potassium ethyl xanthate
-
Neurospora crassa
1.7.1.10
Salicylaldoxime
-
Neurospora crassa
1.7.1.10
Sodium diethyl dithiocarbamate
-
Neurospora crassa
1.7.1.10
sulfite
-
Neurospora crassa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.10
0.07
-
NADH
-
Neurospora crassa
1.7.1.10
0.1
-
NADPH
-
Neurospora crassa
1.7.1.10
3.8
-
hydroxylamine
-
Neurospora crassa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.7.1.10
Neurospora crassa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.7.1.10
partial
Neurospora crassa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.10
additional information
-
360.0 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute
Neurospora crassa
Storage Stability
EC Number
Storage Stability
Organism
1.7.1.10
-15°C, slow loss of activity over several weeks
Neurospora crassa
1.7.1.10
4°C, overnight
Neurospora crassa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.10
hydroxylamine + NADH
no other substances beside nitrite and dinitrobenzene stimulate oxidation of NADPH
394440
Neurospora crassa
NH3 + NAD+ + H2O
-
394440
Neurospora crassa
ir
1.7.1.10
hydroxylamine + NADH
no activity with O-methylhydroxylamine, hydrazine, nitrophenylhydroxylamine and salicylhydroxamic acid
394440
Neurospora crassa
NH3 + NAD+ + H2O
-
394440
Neurospora crassa
ir
1.7.1.10
NO2- + NADH
-
394440
Neurospora crassa
NH3 + H2O + NAD+
-
394440
Neurospora crassa
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.7.1.10
50
-
5 min, stable
Neurospora crassa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.7.1.10
7.5
9.5
highest stability
Neurospora crassa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.10
FAD
stimulates
Neurospora crassa
1.7.1.10
NADH
-
Neurospora crassa
1.7.1.10
NADPH
-
Neurospora crassa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.10
FAD
stimulates
Neurospora crassa
1.7.1.10
NADH
-
Neurospora crassa
1.7.1.10
NADPH
-
Neurospora crassa
General Stability (protein specific)
EC Number
General Stability
Organism
1.7.1.10
dialysis against 3 mM sodium diphosphate, 1 mM cysteine solution, pH 8, 2 h, less than 50% loss of activity, against 10 mM phosphate, pH 8, 1 h, complete loss of activity
Neurospora crassa
1.7.1.10
little or no activity in phosphate buffer unless 1 mM EDTA is included in the reaction mixture
Neurospora crassa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.10
2,2'-dipyridyl
-
Neurospora crassa
1.7.1.10
8-hydroxyquinoline
-
Neurospora crassa
1.7.1.10
Cu2+
-
Neurospora crassa
1.7.1.10
KCN
0.1 mM, complete inhibition
Neurospora crassa
1.7.1.10
NaN3
-
Neurospora crassa
1.7.1.10
o-phenanthroline
-
Neurospora crassa
1.7.1.10
potassium ethyl xanthate
-
Neurospora crassa
1.7.1.10
Salicylaldoxime
-
Neurospora crassa
1.7.1.10
Sodium diethyl dithiocarbamate
-
Neurospora crassa
1.7.1.10
sulfite
-
Neurospora crassa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.10
0.07
-
NADH
-
Neurospora crassa
1.7.1.10
0.1
-
NADPH
-
Neurospora crassa
1.7.1.10
3.8
-
hydroxylamine
-
Neurospora crassa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.10
partial
Neurospora crassa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.10
additional information
-
360.0 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute
Neurospora crassa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.7.1.10
-15°C, slow loss of activity over several weeks
Neurospora crassa
1.7.1.10
4°C, overnight
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.7.1.10
hydroxylamine + NADH
no other substances beside nitrite and dinitrobenzene stimulate oxidation of NADPH
394440
Neurospora crassa
NH3 + NAD+ + H2O
-
394440
Neurospora crassa
ir
1.7.1.10
hydroxylamine + NADH
no activity with O-methylhydroxylamine, hydrazine, nitrophenylhydroxylamine and salicylhydroxamic acid
394440
Neurospora crassa
NH3 + NAD+ + H2O
-
394440
Neurospora crassa
ir
1.7.1.10
NO2- + NADH
-
394440
Neurospora crassa
NH3 + H2O + NAD+
-
394440
Neurospora crassa
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.7.1.10
50
-
5 min, stable
Neurospora crassa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.7.1.10
7.5
9.5
highest stability
Neurospora crassa