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Literature summary extracted from

  • Hirasawa, M.; de Best, J.H.; Knaff, D.B.
    The effect of lysine- and arginine-modifying reagents on spinach ferredoxin:nitrite oxidoreductase (1993), Biochim. Biophys. Acta, 1140, 304-312.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 N-acetylsuccinimide loss of enzymatic activity when reduced ferredoxin serves as electron donor, but very little effect with methyl viologen as electron donor, ferredoxin protects the enzyme Spinacia oleracea
1.7.7.1 Phenylglyoxal loss of enzymatic activity when reduced ferredoxin serves as electron donor, but very little effect with methyl viologen as electron donor, ferredoxin protects the enzyme Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.1
-
 methyl viologen
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 62000
-
 SDS-PAGE Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.7.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 90
-
 methyl viologen as electron donor Spinacia oleracea
1.7.7.1 120
-
 ferredoxin as electron donor Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 nitrite + reduced methyl viologen
-
 Spinacia oleracea NH3 + H2O + oxidized methyl viologen
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.1 7.7
-
-
 Spinacia oleracea