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Literature summary extracted from

  • Porter, D.J.T.; Bright, H.J.
    Propionate-3-nitronate oxidase from Penicillium atrovenetum is a flavoprotein which initiates the autoxidation of its substrate by O2 (1987), J. Biol. Chem., 262, 14428-14434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.3.5 propane-2-nitronate increases the enzyme activity over 40fold when added to a reaction mixture that contains propionate-3-nitronate, the stimulation can be partially inhibited by superoxide dismutase Penicillium atrovenetum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.3.5 soluble
-
 Penicillium atrovenetum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.5 38000
-
 2 * 38000, SDS-PAGE in presence of mercaptoethanol Penicillium atrovenetum
1.7.3.5 73000
-
 gel filtration Penicillium atrovenetum

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.5 Penicillium atrovenetum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.5 homogeneity Penicillium atrovenetum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.3.5 3-aci-nitropropanoate + O2 + H2O = 3-oxopropanoate + nitrite + H2O2 primary products of the enzymatic reaction probably is the nitropropanoate free radical and superoxide Penicillium atrovenetum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.3.5 mycelium
-
 Penicillium atrovenetum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.5 160
-
 butyrate-4-nitronate as substrate Penicillium atrovenetum
1.7.3.5 274
-
 propionate-3-nitronate as substrate Penicillium atrovenetum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.5 3-aci-nitropropanoate + O2
-
 Penicillium atrovenetum 3-oxopropanoate + nitrite + H2O2
-
 ?
1.7.3.5 butyrate-4-nitronate + O2
-
 Penicillium atrovenetum succinate + nitrite + H2O2
-
 ?
1.7.3.5 additional information neither cytochrome c nor K3Fe(CN)6 serve as oxidants, other substrates tested are oxidized less than 2% the rate of 3-nitropropanoate Penicillium atrovenetum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.5 dimer 2 * 38000, SDS-PAGE in presence of mercaptoethanol Penicillium atrovenetum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.5 25
-
 assay at Penicillium atrovenetum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.3.5 350
-
 3-aci-Nitropropanoate
-
 Penicillium atrovenetum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.5 8.1
-
 assay at Penicillium atrovenetum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.5 FMN flavoprotein Penicillium atrovenetum