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Literature summary extracted from

  • Chen, S.; Clarke, P.E.; Martino, P.A.; Deng, P.S.K.; Yeh, C.H.; Lee, T.D.; Prochaska, H.J.; Talalay, P.
    Mouse liver NAD(P)H:quinone acceptor oxidoreductase: protein sequence analysis by tandem mass spectrometry, cDNA cloning, expression in Escherichia coli, and enzyme activity analysis (1994), Protein Sci., 3, 1296-1304.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 expression in Escherichia coli Mus musculus
1.6.5.2 expression in Escherichia coli Homo sapiens
1.6.5.2 expression in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Homo sapiens
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin) competitive vs. NADH Mus musculus
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Rattus norvegicus
1.6.5.2 5,7-dihydroxyflavone 0.009 mM, approx. 90% inhibition Homo sapiens
1.6.5.2 5,7-dihydroxyflavone 0.009 mM, approx. 20% inhibition Mus musculus
1.6.5.2 5,7-dihydroxyflavone 0.009 mM, approx. 80% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.002
-
 menadione
-
 Homo sapiens
1.6.5.2 0.0026
-
 menadione
-
 Rattus norvegicus
1.6.5.2 0.0041
-
 menadione
-
 Mus musculus
1.6.5.2 0.12
-
 NADPH
-
 Rattus norvegicus
1.6.5.2 0.17
-
 NADPH
-
 Mus musculus
1.6.5.2 0.19
-
 NADH
-
 Rattus norvegicus
1.6.5.2 0.24
-
 NADPH
-
 Homo sapiens
1.6.5.2 0.29
-
 NADH
-
 Homo sapiens
1.6.5.2 0.32
-
 NADH
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 30835
-
 2 * 30835, electrospray mass spectrometry Mus musculus
1.6.5.2 30835
-
 2 * 30835, electrospray mass spectrometry Homo sapiens
1.6.5.2 30835
-
 2 * 30835, electrospray mass spectrometry Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Homo sapiens
-
-
-
1.6.5.2 Mus musculus
-
 2 forms have the same primary sequence
-
1.6.5.2 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.6.5.2 no glycoprotein
-
 Rattus norvegicus
1.6.5.2 no glycoprotein very unstable posttranslational modification is suggested that is not detectable by mass spectrometry Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2 recombinant enzyme Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H
-
 Mus musculus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
1.6.5.2 menadione + NADH + H+
-
 Mus musculus menadiol + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.5.2 dimer 2 * 30835, electrospray mass spectrometry Mus musculus
1.6.5.2 dimer 2 * 30835, electrospray mass spectrometry Homo sapiens
1.6.5.2 dimer 2 * 30835, electrospray mass spectrometry Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD
-
 Homo sapiens
1.6.5.2 FAD
-
 Rattus norvegicus
1.6.5.2 FAD purified recombinant enzyme contains FAD whereas purified native enzyme does not contain FAD Mus musculus