Purification of hydroxylamine oxidase from Thiosphaera pantotropha. Identification of electron acceptors that couple heterotrophic nitrification to aerobic denitrification
Wehrfritz, J.M.; Reilly, A.; Spiro, S.; Richardson, D.J.; FEBS Lett. 335, 246-250 (1993) 
Data extracted from this reference:
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.3.6
0.01
-
ferricytochrome c551
pH 7.8, 25°C
Paracoccus denitrificans
1.7.3.6
0.033
-
pseudoazurin
pH 7.8, 25°C
Paracoccus denitrificans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.7.3.6
periplasm
-
Paracoccus denitrificans
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.7.3.6
Fe
non-heme iron enzyme, the enzyme could possess a non-heme ferric iron centre that is lost when the 20000 Da polypeptide is subjected to SDS-PAGE, but that can be reconstituted by addition of Fe3+ ions to the refolded enzyme
Paracoccus denitrificans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.3.6
20000
-
x * 20000, SDS-PAGE
Paracoccus denitrificans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.7.3.6
Paracoccus denitrificans
-
i.e. Thiosphaera pantotropha, heterotrophic nitrifying bacterium
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.7.3.6
partial
Paracoccus denitrificans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.7.3.6
hydroxylamine + ferricytochrome c551
-
394345
Paracoccus denitrificans
nitroxyl + ferrocytochrome c551 + H+
-
-
-
?
1.7.3.6
hydroxylamine + pseudoazurin
-
394345
Paracoccus denitrificans
nitrite + reduced pseudoazurin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.7.3.6
?
x * 20000, SDS-PAGE
Paracoccus denitrificans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.3.6
additional information
non-heme iron enzyme
Paracoccus denitrificans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.3.6
additional information
non-heme iron enzyme
Paracoccus denitrificans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.3.6
0.01
-
ferricytochrome c551
pH 7.8, 25°C
Paracoccus denitrificans
1.7.3.6
0.033
-
pseudoazurin
pH 7.8, 25°C
Paracoccus denitrificans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.7.3.6
periplasm
-
Paracoccus denitrificans
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.7.3.6
Fe
non-heme iron enzyme, the enzyme could possess a non-heme ferric iron centre that is lost when the 20000 Da polypeptide is subjected to SDS-PAGE, but that can be reconstituted by addition of Fe3+ ions to the refolded enzyme
Paracoccus denitrificans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.3.6
20000
-
x * 20000, SDS-PAGE
Paracoccus denitrificans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.3.6
partial
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.7.3.6
hydroxylamine + ferricytochrome c551
-
394345
Paracoccus denitrificans
nitroxyl + ferrocytochrome c551 + H+
-
-
-
?
1.7.3.6
hydroxylamine + pseudoazurin
-
394345
Paracoccus denitrificans
nitrite + reduced pseudoazurin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.7.3.6
?
x * 20000, SDS-PAGE
Paracoccus denitrificans