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Literature summary extracted from

  • Schalk, J.; de Vries, S.; Kuenen, J.G.; Jetten, M.S.
    Involvement of a novel hydroxylamine oxidoreductase in anaerobic ammonium oxidation (2000), Biochemistry, 39, 5405-5412.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.8 cyanide addition of 0.035 mM, 0.085 mM, or 0.35 mM cyanide results in 25%, 50%, and 95% inhibition, respectively Candidatus Brocadia anammoxidans
1.7.2.8 H2O2 0.0004 mM, 0.004 mM, and 0.04 mM results in a loss of activity of 30%, 75%, and 90%, respectively Candidatus Brocadia anammoxidans
1.7.2.8 hydrazine competes strongly with hydroxylamine. Addition of 0.015 mM, 0.035 mM, and 0.35 mM results in 15%, 50%, and 85% inhibition, respectively Candidatus Brocadia anammoxidans
1.7.2.8 additional information sodium azide, methanol, sodium nitrite, and the chelating agent EDTA do not inhibit enzyme activity Candidatus Brocadia anammoxidans
1.7.2.8 SDS activity in the presence of 0.75%, 3%, and 8% SDS is reduced by 20%, 50%, and 95%, respectively Candidatus Brocadia anammoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.8 0.018
-
 hydrazine pH 8.0, 35°C, reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate Candidatus Brocadia anammoxidans
1.7.2.8 0.026
-
 hydroxylamine pH 8.0, 35°C, reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate Candidatus Brocadia anammoxidans
1.7.2.8 0.127
-
 hydroxylamine pH 8.0, 65°C, reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate Candidatus Brocadia anammoxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.8 58000
-
 2 * 58000, SDS-PAGE Candidatus Brocadia anammoxidans
1.7.2.8 150000
-
 gel filtration Candidatus Brocadia anammoxidans
1.7.2.8 183000
-
 nondenaturing PAGE Candidatus Brocadia anammoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.8 additional information Candidatus Brocadia anammoxidans the enzyme is involved in the pathway of anaerobic ammonium oxidation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.8 Candidatus Brocadia anammoxidans
-
 hydroxylamine oxidoreductase is purified and characterized from an anaerobic ammonium-oxidizing (Anammox) enrichment culture
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.8
-
 Candidatus Brocadia anammoxidans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.8 hydrazine + 2,6-dichlorophenolindophenol
-
 Candidatus Brocadia anammoxidans N2 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.7.2.8 hydrazine + ferricyanide ferricyanide as the electron acceptor results in the highest specific activity. Hydrazine is solely converted to N2 Candidatus Brocadia anammoxidans N2 + ferrocyanide
-
 ?
1.7.2.8 hydrazine + ferricytochrome horse heart cytochrome. Hydrazine is solely converted to N2 Candidatus Brocadia anammoxidans N2 + ferrocytochrome
-
 ?
1.7.2.8 hydrazine + methylthiazolyltetrazolium bromide reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate. Hydrazine is solely converted to N2 Candidatus Brocadia anammoxidans N2 + reduced methylthiazolyltetrazolium bromide
-
 ?
1.7.2.8 hydroxylamine + 2,6-dichlorophenolindophenol horse heart cytochrome. The conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected Candidatus Brocadia anammoxidans N2O + reduced 2,6-dichlorophenolindophenol small amounts of NO are also formed ?
1.7.2.8 hydroxylamine + ferricyanide ferricyanide as the electron acceptor results in the highest specific activity. The conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected Candidatus Brocadia anammoxidans N2O + ferrocyanide small amounts of NO are also formed ?
1.7.2.8 hydroxylamine + ferricytochrome horse heart cytochrome. The conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected Candidatus Brocadia anammoxidans N2O + ferrocytochrome small amounts of NO are also formed ?
1.7.2.8 hydroxylamine + methylthiazolyltetrazolium bromide the conversion of hydroxylamine by the enzyme results in the formation of NO and N2O. A small amount of NO2- is measured, but this is probably due to the chemical reaction of NO. No other nitrogen compounds are detected Candidatus Brocadia anammoxidans N2O + reduced methylthiazolyltetrazolium bromide small amounts of NO are also formed ?
1.7.2.8 additional information the enzyme is involved in the pathway of anaerobic ammonium oxidation Candidatus Brocadia anammoxidans ?
-
 ?
1.7.2.8 additional information methanol is not a substrate, no activity with NAD+, benzyl viologen and Wurster's blue. The enzyme from anaerobic ammonium-oxidizing (Anammox) enrichment culture is also capable of reducing NO to N2O with a specific activity of 2.9 units/mg at room temperature. Reduction of nitrite in the presence of reduced methyl viologen results in the production of N2O and some NO (below 5%). No other nitrogen compounds are detected. In the presence of ascorbate and PMS, no nitrite conversion is observed Candidatus Brocadia anammoxidans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.8 dimer 2 * 58000, SDS-PAGE Candidatus Brocadia anammoxidans

Synonyms

EC Number Synonyms Comment Organism
1.7.2.8 HAO
-
 Candidatus Brocadia anammoxidans
1.7.2.8 hydroxylamine oxidoreductase
-
 Candidatus Brocadia anammoxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.8 35
-
 assay at Candidatus Brocadia anammoxidans
1.7.2.8 65
-
 highest activity with hydroxylamine as substrate Candidatus Brocadia anammoxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.8 8
-
-
 Candidatus Brocadia anammoxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.8 heme 1 mol of enzyme contains 26 mol hemes Candidatus Brocadia anammoxidans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.7.2.8 Candidatus Brocadia anammoxidans isoelectric focusing
-
 5.5