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Literature summary extracted from

  • Zimmermann, T.; Gasser, F.; Kulla, H.G.; Leisinger, T.
    Comparison of two bacterial azoreductases acquired during adaptation to growth on azo dyes (1984), Arch. Microbiol., 138, 37-43.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 Orange I substrate inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.0011
-
 NADPH
-
 Pseudomonas sp.
1.7.1.6 0.0024
-
 1-(4'-carboxyphenylazo)-4-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.0026
-
 1-(4'-sulfophenylazo)-4-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.04
-
 1-(4'-sulfophenyl-N-methylhydrazo)-4-naphthol
-
 Pseudomonas sp.
1.7.1.6 0.054
-
 NADH
-
 Pseudomonas sp.
1.7.1.6 0.204
-
 1-(4'-sulfophenylazo)-4-anthranol
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 20200
-
 1 * 20200, strain K24, SDS-PAGE, Orange I azoreductase Pseudomonas sp.
1.7.1.6 21500
-
 strain KF 24, gel filtration, Orange I azoreductase Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Pseudomonas sp. key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Pseudomonas sp. K24 key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Pseudomonas sp.
-
-
-
1.7.1.6 Pseudomonas sp. K24
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 2.87
-
-
 Pseudomonas sp.
1.7.1.6 2868
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H
-
 Pseudomonas sp. 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H
-
 Pseudomonas sp. K24 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene
-
 ?
1.7.1.6 1-(4'-sulfophenyl-N-methylhydrazo)-4-naphthol + NADH + H+ i.e. carboxy-Orange I Pseudomonas sp. ?
-
 r
1.7.1.6 1-(4'-sulfophenylazo)-4-anthranol + NADH + H+
-
 Pseudomonas sp. ?
-
 r
1.7.1.6 1-(4'-sulfophenylazo)-4-naphthol + NADH + H+
-
 Pseudomonas sp. ?
-
 r
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Orange I azoreductase: absolute requirement for a hydroxyl group in 4'-position of the naphthol ring of the substrate molecule Pseudomonas sp. N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Orange II azoreductase: requirement for substrates with a 2-naphthol moiety Pseudomonas sp. N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures Pseudomonas sp. N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Orange I azoreductase: absolute requirement for a hydroxyl group in 4'-position of the naphthol ring of the substrate molecule Pseudomonas sp. K24 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH Orange II azoreductase: requirement for substrates with a 2-naphthol moiety Pseudomonas sp. K24 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures Pseudomonas sp. K24 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-[(4-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H trivial name Orange I Pseudomonas sp. 4-amino-benzenesulfonic acid + 1-amino-4-hydroxynaphthalene + NADP+
-
 ?
1.7.1.6 4-[(4-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H trivial name Orange I Pseudomonas sp. K24 4-amino-benzenesulfonic acid + 1-amino-4-hydroxynaphthalene + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 monomer 1 * 20200, strain K24, SDS-PAGE, Orange I azoreductase Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 Orange I azoreductase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 41
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 6.2
-
 Orange I Pseudomonas sp.
1.7.1.6 6.8
-
 carboxy-Orange I Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 NADH
-
 Pseudomonas sp.
1.7.1.6 NADPH
-
 Pseudomonas sp.