BRENDA - Enzyme Database

Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46

Zimmermann, T.; Kulla, H.G.; Leisinger, T.; Eur. J. Biochem. 129, 197-203 (1982) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.6
Orange II
substrate inhibition at high concentration
Pseudomonas sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.6
0.0009
-
1-phenylazo-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.001
-
1-(4'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0013
-
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0014
-
1-phenylazo-2-hydroxy-6-carboxynaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0015
-
1-(3'-carboxyphenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0018
-
1-(3'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0018
-
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.005
-
NADPH
-
Pseudomonas sp.
1.7.1.6
0.0052
-
1-(4'-phosphophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.008
-
1-(4'-carboxyphenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0105
-
1-(3'-phosphophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.013
-
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0132
-
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0137
-
1-(2'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0148
-
1-(4'-sulfoaminophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.017
-
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0198
-
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.18
-
NADH
-
Pseudomonas sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.6
30000
-
1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase
Pseudomonas sp.
1.7.1.6
32000
-
strain KF 46, gel filtration, Orange II azoreductase
Pseudomonas sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.7.1.6
Pseudomonas sp.
-
Orange II azoreductase induced by both Orange II and carboxy-Orange II
-
1.7.1.6
Pseudomonas sp. KF46
-
Orange II azoreductase induced by both Orange II and carboxy-Orange II
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.7.1.6
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.6
2868
-
orange I azoreductase, strain KF24
Pseudomonas sp.
1.7.1.6
17830
-
orange II azoreductase, strain KF46
Pseudomonas sp.
Storage Stability
EC Number
Storage Stability
Organism
1.7.1.6
-20°C, 50% glycerol, several months
Pseudomonas sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.7.1.6
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol + NADH + H+
-
394298
Pseudomonas sp.
4-amino-2-methyl-1-benzenesulfonic acid + NAD+ + 1-amino-2-hydroxynaphthalene
-
-
-
r
1.7.1.6
1-(2'-methyl-4'-sulfophenylazo)2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-2-methyl-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(2'-sulfophenylazo)-2-naphthol + NADH + H+
-
394298
Pseudomonas sp.
?
-
-
-
r
1.7.1.6
1-(3'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
3-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(3'-phosphophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
3-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(3'-sulfophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
3-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene + NADH + H+
-
394298
Pseudomonas sp.
?
-
-
-
r
1.7.1.6
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-carboxyphenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp. KF46
4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-naphthol + NADH + H+
i.e. carboxy-Orange II
394298
Pseudomonas sp.
4-aminobenzoate + NAD+ + ?
-
-
-
r
1.7.1.6
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-nitrophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp. KF46
4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H
-
394298
Pseudomonas sp. KF46
4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-phenylazo-2-hydroxy-6-carboxynaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-phenylazo-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
4-(dimethylamino)azobenzene + 2 NADPH
Orange II azoreductase: requirement for substrates with a 2-naphthol moiety
394298
Pseudomonas sp.
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
394298
Pseudomonas sp.
?
1.7.1.6
4-(dimethylamino)azobenzene + 2 NADPH
Orange II azoreductase: requirement for substrates with a 2-naphthol moiety
394298
Pseudomonas sp. KF46
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H
trivial name Orange II
394298
Pseudomonas sp.
4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H
trivial name Orange II
394298
Pseudomonas sp. KF46
4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
Solar Orange + NADPH
-
394298
Pseudomonas sp.
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.7.1.6
monomer
1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase
Pseudomonas sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.7.1.6
45
-
-
Pseudomonas sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.7.1.6
20
50
20 C: about 25% activity with carboxy-Orange II, about 30% activity with Orange II, 50 C: about 95% activity with carboxy-Orange II, about 80% activity with Orange II
Pseudomonas sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.7.1.6
35
-
stable below
Pseudomonas sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.1.6
5
8
Orange II + NADH
Pseudomonas sp.
1.7.1.6
6.5
-
carboxy-Orange II + NADH
Pseudomonas sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.6
NADH
-
Pseudomonas sp.
1.7.1.6
NADPH
-
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.6
NADH
-
Pseudomonas sp.
1.7.1.6
NADPH
-
Pseudomonas sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.6
Orange II
substrate inhibition at high concentration
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.6
0.0009
-
1-phenylazo-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.001
-
1-(4'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0013
-
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0014
-
1-phenylazo-2-hydroxy-6-carboxynaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0015
-
1-(3'-carboxyphenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0018
-
1-(3'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0018
-
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.005
-
NADPH
-
Pseudomonas sp.
1.7.1.6
0.0052
-
1-(4'-phosphophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.008
-
1-(4'-carboxyphenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0105
-
1-(3'-phosphophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.013
-
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0132
-
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0137
-
1-(2'-sulfophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.0148
-
1-(4'-sulfoaminophenylazo)-2-naphthol
-
Pseudomonas sp.
1.7.1.6
0.017
-
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.0198
-
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene
-
Pseudomonas sp.
1.7.1.6
0.18
-
NADH
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.6
30000
-
1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase
Pseudomonas sp.
1.7.1.6
32000
-
strain KF 46, gel filtration, Orange II azoreductase
Pseudomonas sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.6
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.7.1.6
2868
-
orange I azoreductase, strain KF24
Pseudomonas sp.
1.7.1.6
17830
-
orange II azoreductase, strain KF46
Pseudomonas sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.7.1.6
-20°C, 50% glycerol, several months
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.7.1.6
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol + NADH + H+
-
394298
Pseudomonas sp.
4-amino-2-methyl-1-benzenesulfonic acid + NAD+ + 1-amino-2-hydroxynaphthalene
-
-
-
r
1.7.1.6
1-(2'-methyl-4'-sulfophenylazo)2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-2-methyl-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(2'-sulfophenylazo)-2-naphthol + NADH + H+
-
394298
Pseudomonas sp.
?
-
-
-
r
1.7.1.6
1-(3'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
3-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(3'-phosphophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
3-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(3'-sulfophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
3-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene + NADH + H+
-
394298
Pseudomonas sp.
?
-
-
-
r
1.7.1.6
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-carboxyphenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp. KF46
4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
1-(4'-carboxyphenylazo)-2-naphthol + NADH + H+
i.e. carboxy-Orange II
394298
Pseudomonas sp.
4-aminobenzoate + NAD+ + ?
-
-
-
r
1.7.1.6
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-nitrophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-phosphophenylazo)-2-naphthol + NAD(P)H
-
394298
Pseudomonas sp. KF46
4-amino-1-benzenephosphate + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H
-
394298
Pseudomonas sp.
4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-sulfonaminophenylazo-)2-naphthol + NAD(P)H
-
394298
Pseudomonas sp. KF46
4-amino-1-benzenesulfonamine + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
4-sulfophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-phenylazo-2-hydroxy-6-carboxynaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-carboxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
1-phenylazo-2-hydroxy-6-sulfonaphthalene + NAD(P)H
-
394298
Pseudomonas sp.
phenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
4-(dimethylamino)azobenzene + 2 NADPH
Orange II azoreductase: requirement for substrates with a 2-naphthol moiety
394298
Pseudomonas sp.
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
394298
Pseudomonas sp.
?
1.7.1.6
4-(dimethylamino)azobenzene + 2 NADPH
Orange II azoreductase: requirement for substrates with a 2-naphthol moiety
394298
Pseudomonas sp. KF46
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H
trivial name Orange II
394298
Pseudomonas sp.
4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp.
?
1.7.1.6
4-[(2-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H
trivial name Orange II
394298
Pseudomonas sp. KF46
4-amino-1-benzenesulfonic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene
-
394298
Pseudomonas sp. KF46
?
1.7.1.6
Solar Orange + NADPH
-
394298
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.7.1.6
monomer
1 * 30000, strain KF46, SDS-PAGE, Orange II azoreductase
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.7.1.6
45
-
-
Pseudomonas sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.7.1.6
20
50
20 C: about 25% activity with carboxy-Orange II, about 30% activity with Orange II, 50 C: about 95% activity with carboxy-Orange II, about 80% activity with Orange II
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.7.1.6
35
-
stable below
Pseudomonas sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.1.6
5
8
Orange II + NADH
Pseudomonas sp.
1.7.1.6
6.5
-
carboxy-Orange II + NADH
Pseudomonas sp.