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Literature summary extracted from

  • Matsudomi, N.; Kobayashi, K.; Akuta, S.
    Purification and some properties of new coccine (NC)-reductase from Bacillus cereus T-105 strain (1977), Agric. Biol. Chem., 41, 2323-2329.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.6 FMN increases activity Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.6 Ag+ 1 mM, 92% inhibition Bacillus cereus
1.7.1.6 Cu2+ 1 mM, 88% inhibition Bacillus cereus
1.7.1.6 Fe2+ 1 mM, 90% inhibition Bacillus cereus
1.7.1.6 Fe3+ 1 mM, 85% inhibition Bacillus cereus
1.7.1.6 Hg2+ 1 mM, 75% inhibition Bacillus cereus
1.7.1.6 additional information not inhibited by N-ethylmaleimide, p-chloromercuribenzoate and monoiodoacetic acid Bacillus cereus
1.7.1.6 SDS 1 mM, 50% inhibition Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.6 intracellular
-
 Bacillus cereus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.6 Mg2+ slight activation Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Bacillus cereus
-
 T-105
-
1.7.1.6 Bacillus cereus T-105
-
 T-105
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6
-
 Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 0.566
-
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Bacillus cereus N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 4-(dimethylamino)azobenzene + 2 NADPH
-
 Bacillus cereus T-105 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+
-
 ?
1.7.1.6 new coccine + NADPH
-
 Bacillus cereus ?
-
 ?
1.7.1.6 new coccine + NADPH
-
 Bacillus cereus T-105 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 40
-
-
 Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.1.6 5 70 at 5°C and 70°C: about 55% of maximal activity Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.6 60
-
 10 min, stable up to Bacillus cereus
1.7.1.6 80
-
 10 min, 45% loss of activity Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 7
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.6 4 10 pH 4.0: about 10% activity, pH 10.0: about 20% activity Bacillus cereus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.6 6 8 5°C, 7 days, stable Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FAD increases activity Bacillus cereus
1.7.1.6 flavin prosthetic group seems to be flavin Bacillus cereus
1.7.1.6 additional information no activity with NADH Bacillus cereus
1.7.1.6 NADPH specific for Bacillus cereus
1.7.1.6 riboflavin no activation Bacillus cereus