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Literature summary extracted from

  • Mackenzie, J.J.; Sorensen, L.B.
    Guanosine 5-phosphate reductase of human erythrocytes (1973), Biochim. Biophys. Acta, 327, 282-294.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.7 2-mercaptoethanol activation Homo sapiens
1.7.1.7 dithiothreitol activation Homo sapiens
1.7.1.7 thioglycolic acid activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.7 5,5'-dithiobis(2-nitrobenzoate) 0.66 mM, 33% inhibition after 30 min Homo sapiens
1.7.1.7 Ca2+ 10 mM, 54% inhibition Homo sapiens
1.7.1.7 Cu2+
-
 Homo sapiens
1.7.1.7 Fe2+ 10 mM, more than 90% inhibition Homo sapiens
1.7.1.7 IMP
-
 Homo sapiens
1.7.1.7 Mg2+ 10 mM, more than 90% inhibition, 1 mM, 12% inhibition Homo sapiens
1.7.1.7 Mn2+
-
 Homo sapiens
1.7.1.7 Ni2+
-
 Homo sapiens
1.7.1.7 p-hydroxymercuribenzoate 0.66 mM, 18% inhibition after 30 min Homo sapiens
1.7.1.7 XMP
-
 Homo sapiens
1.7.1.7 Zn2+ 10 mM, more than 90% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.7 0.0049
-
 GMP
-
 Homo sapiens
1.7.1.7 0.0085
-
 NADPH
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.7 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.1.7 IMP + NH3 + NADP+ = GMP + NADPH + H+ first order reaction Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.7 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.7 0.0052
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.7.1.7 -70°C, 2 months, less than 20% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.7 NADPH + guanosine 5'-phosphate
-
 Homo sapiens NADP+ + inosine 5'-phosphate + NH3
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.7 67
-
 15 min, 40% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.7 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.1.7 6 9 pH 6: about 50% of activity at maximum, pH 9: about 70% of activity at maximum Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.7 0.0011
-
 XMP
-
 Homo sapiens