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Literature summary extracted from

  • Zhang, M.; Scott, J.G.
    Purification and characterization of cytochrome b5 reductase from the house fly, Musca domestica (1996), Comp. Biochem. Physiol. B, 113, 175-183.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.013
-
 ferricytochrome b5
-
 Musca domestica
1.6.2.2 0.017
-
 ferricyanide
-
 Musca domestica
1.6.2.2 0.044
-
 NADH
-
 Musca domestica
1.6.2.2 0.83
-
 oxidized 2,6-dichlorophenolindophenol
-
 Musca domestica
1.6.2.2 14
-
 ferricytochrome b5
-
 Musca domestica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 endoplasmic reticulum
-
 Musca domestica 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 31000
-
 x * 31000, may occur from protease cleavage of the 33000 Da protein during purification Musca domestica
1.6.2.2 33000
-
 x * 33000, SDS-PAGE Musca domestica

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Musca domestica
-
 learn-pyrethroid-resistance strain, house fly
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 Triton X-100, DEAE-cellulose, CM-cellulose, 5'-ADP-agarose Musca domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 2.042
-
 reduction of cytochrome b5 Musca domestica
1.6.2.2 1007
-
 reduction of ferricyanide Musca domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH uses both NADH and NADPH as electron donors, artificial acceptors: ferricyanide, 2,6-dichlophenolindophenol Musca domestica 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricyanide
-
 Musca domestica NAD+ + H+ + ferrocyanide
-
 r
1.6.2.2 NADH + ferricytochrome b5
-
 Musca domestica NAD+ + H+ + ferrocytochrome b5
-
 r
1.6.2.2 NADH + oxidized 2,6-dichlorophenolindophenol
-
 Musca domestica NAD+ + H+ + reduced 2,6-dichlorophenolindophenol
-
 r
1.6.2.2 NADPH + ferricytochrome b5
-
 Musca domestica NADP+ + H+ + ferrocytochrome b5
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 33000, SDS-PAGE Musca domestica
1.6.2.2 ? x * 31000, may occur from protease cleavage of the 33000 Da protein during purification Musca domestica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 50.2
-
 2,6-dichlorophenolindophenol
-
 Musca domestica
1.6.2.2 297
-
 NADPH
-
 Musca domestica
1.6.2.2 307
-
 ferricyanide
-
 Musca domestica
1.6.2.2 332
-
 NADH
-
 Musca domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADPH
-
 Musca domestica