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Literature summary extracted from
- High-level expression in Escherichia coli of the soluble, catalytic domain of rat hepatic cytochrome b5 reductase (1996), Protein Expr. Purif., 8, 41-47.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | expression in Escherichia coli | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.2 | 0.006 | - |
NADH | recombinant enzyme | Rattus norvegicus |  |
| 1.6.2.2 | 0.007 | - |
ferricyanide | recombinant enzyme | Rattus norvegicus | |
| 1.6.2.2 | 0.011 | - |
ferricytochrome b5 | recombinant enzyme | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 30000 | - |
1 * 30000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
| 1.6.2.2 | 30588 | - |
1 * 30588, recombinant enzyme, MALDI-TOF mass spectrometry | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | recombinant enzyme, affinity chromatography on 5'-ADP-agarose | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 1400 | - |
recombinant enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.2 | 2 ferricytochrome b5 + NADH | recombinant enzyme, very low activity with NADPH | Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 1.6.2.2 | NADH + ferricytochrome b5 | - |
Rattus norvegicus | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.2 | monomer | 1 * 30000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
| 1.6.2.2 | monomer | 1 * 30588, recombinant enzyme, MALDI-TOF mass spectrometry | Rattus norvegicus |
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Release 2023.1 (January 2023)
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