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Literature summary extracted from

  • Mihara, K.; Sato, R.
    Detergent-solubilized NADH-cytochrome b5 reductase (1978), Methods Enzymol., 52, 102-108.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.2 0.2% phosphatidycholine liposomes or Triton X-100 stabilize Oryctolagus cuniculus
1.6.2.2 freezing/thawing: 50% inactivation Oryctolagus cuniculus
1.6.2.2 Triton X-100 stabilizes Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 endoplasmic reticulum
-
 Oryctolagus cuniculus 5783
-
1.6.2.2 membrane enzyme binds in vitro to a variety of membrane preparations, protease treatment removes a hydrophobic segment that is responsible for membrane binding Oryctolagus cuniculus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 33000
-
 x * 33000, SDS-PAGE, gel filtration in the presence of 6 M guanidine hydrochloride, gel filtration in the presence of 0.5% sodium deoxycholate Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 622.5
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 -70°C, concentrated solution, several months, no loss of activity Oryctolagus cuniculus
1.6.2.2 -70°C, several months, no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH low activity with trypsin-solubilized cytochrome b5 Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricyanide
-
 Oryctolagus cuniculus NAD+ + H+ + ferrocyanide
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 ? x * 33000, SDS-PAGE, gel filtration in the presence of 6 M guanidine hydrochloride, gel filtration in the presence of 0.5% sodium deoxycholate Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 667
-
 ferricyanide
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Oryctolagus cuniculus