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Literature summary extracted from
- Purification and characterization of microsomal cytochrome b5 and NADH cytochrome b5 reductase from Pisum sativum (1987), Plant Physiol., 85, 457-462.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.2 | 0.000007 | - |
ferricytochrome b5 | - |
Pisum sativum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.6.2.2 | endoplasmic reticulum | - |
Pisum sativum | 5783 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.2 | additional information | rate of reduction depends on ionic strength | Pisum sativum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 34500 | - |
4 * 34500, SDS-PAGE | Pisum sativum |
| 1.6.2.2 | 135000 | - |
gel filtration | Pisum sativum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | Pisum sativum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | - |
Pisum sativum |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.6.2.2 | reconstitution | Pisum sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.6.2.2 | seedling | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 0.18 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.2.2 | NADH + ferricyanide | - |
Pisum sativum | NAD+ + H+ + ferrocyanide | - |
r | |
| 1.6.2.2 | NADH + ferricytochrome b5 | - |
Pisum sativum | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.6.2.2 | tetramer | 4 * 34500, SDS-PAGE | Pisum sativum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.6.2.2 | 367 | - |
ferricyanide | - |
Pisum sativum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.2.2 | 5 | 5.7 | - |
Pisum sativum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.2.2 | NADH | - |
Pisum sativum | |
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