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Literature summary extracted from

  • Tamura, M.; Yoshida, S.; Tamura, T.; Saitoh, T.; Takeshita, M.
    Effect of divalent cations on NADH-dependent and NADPH-dependent cytochrome b5 reduction by hepatic microsomes (1990), Arch. Biochem. Biophys., 280, 313-319.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.2.2 Detergents e.g. Triton X-100, activation Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0053
-
ferricytochrome b5 in the presence of 2 mM Ca2+ Sus scrofa
1.6.2.2 0.0112
-
NADH in the presence of 2 mM Ca2+ Sus scrofa
1.6.2.2 0.0136
-
ferricyanide in the presence of 2 mM Ca2+ Sus scrofa
1.6.2.2 0.0195
-
NADH
-
Sus scrofa
1.6.2.2 0.0231
-
ferricyanide
-
Sus scrofa
1.6.2.2 0.0349
-
ferricytochrome b5
-
Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.2.2 Ca2+ 2 mM, 23fold activation Sus scrofa
1.6.2.2 Mg2+ half maximal activation at 1 mM Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 0.0039
-
activity in liver microsomes Sus scrofa
1.6.2.2 0.0648
-
activity in liver microsomes in the presence of 2 mM MgCl2 Sus scrofa
1.6.2.2 0.0888
-
activity in liver microsomes in the presence of 2 mM CaCl2 Sus scrofa
1.6.2.2 0.372
-
in the presence of 0.1% Triton X-100 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 NADH + ferricytochrome b5
-
Sus scrofa NAD+ + H+ + ferrocytochrome b5
-
r
1.6.2.2 NADH + ferricytochrome b5
-
Sus scrofa NAD+ + H+ + 2 ferrocytochrome b5
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
Sus scrofa