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Literature summary extracted from

  • Kahn, K.; Tipton, P.A.
    Kinetic mechanism and cofactor content of soybean root nodule urate oxidase (1997), Biochemistry, 36, 4731-4738.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.3.3 expression in Escherichia coli Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 9-methyluric acid competitive versus urate Glycine max
1.7.3.3 xanthine competitive versus urate, noncompetitive versus O2 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.0098
-
 uric acid
-
 Glycine max
1.7.3.3 0.061
-
 O2
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 35050
-
 deduced from nucleotide sequence of cDNA Glycine max
1.7.3.3 35060
-
 MALDI-TOF mass spectrometry Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Glycine max
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.3.3 root nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 3.6
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
1.7.3.3 4°C, several weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Glycine max 5-hydroxyisourate + H2O2
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.3 additional information no cofactors Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.3.3 additional information
-
 additional information 1-methylurate, 3-methylurate, 7-methylurate do not inhibit significantly Glycine max
1.7.3.3 4.5
-
 xanthine competitive with urate Glycine max