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Literature summary extracted from

  • Rainbird, R.M.; Atkins, C.A.
    Purification and some properties of urate oxidase from nitrogen-fixing nodules of cowpea (1981), Biochim. Biophys. Acta, 659, 132-140.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.3.3 frozen enzyme retains complete activity Vigna unguiculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 2,2'-dipyridyl
-
 Vigna unguiculata
1.7.3.3 adenine
-
 Vigna unguiculata
1.7.3.3 Allantoic acid weak Vigna unguiculata
1.7.3.3 allantoin
-
 Vigna unguiculata
1.7.3.3 CN-
-
 Vigna unguiculata
1.7.3.3 Co2+
-
 Vigna unguiculata
1.7.3.3 Cu2+
-
 Vigna unguiculata
1.7.3.3 EDTA
-
 Vigna unguiculata
1.7.3.3 glutamine
-
 Vigna unguiculata
1.7.3.3 glyoxylic acid weak Vigna unguiculata
1.7.3.3 guanine
-
 Vigna unguiculata
1.7.3.3 hypoxanthine weak Vigna unguiculata
1.7.3.3 inosine 5'-monophosphate
-
 Vigna unguiculata
1.7.3.3 Mg2+ slight Vigna unguiculata
1.7.3.3 Mn2+
-
 Vigna unguiculata
1.7.3.3 NH4+
-
 Vigna unguiculata
1.7.3.3 o-phenanthroline
-
 Vigna unguiculata
1.7.3.3 Salicylhydroxamic acid
-
 Vigna unguiculata
1.7.3.3 Urea weak Vigna unguiculata
1.7.3.3 xanthine
-
 Vigna unguiculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.018
-
 uric acid
-
 Vigna unguiculata
1.7.3.3 0.029
-
 O2
-
 Vigna unguiculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.3.3 Iron Fe3+: stimulation Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 50000
-
 gel filtration Vigna unguiculata
1.7.3.3 100000
-
 gel filtration Vigna unguiculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Vigna unguiculata
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 5.6
-
-
 Vigna unguiculata

Storage Stability

EC Number Storage Stability Organism
1.7.3.3 4°C, 7 days, 40% loss of activity Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Vigna unguiculata 5-hydroxyisourate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.3 dimer at pH 8.8 the enzyme associates to form a dimer of about 10000 Vigna unguiculata
1.7.3.3 monomer native enzyme at pH 7.5 is a monomer with MW 50000 Vigna unguiculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.3 18 20 2 days, 70% loss of activity Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 9 10
-
 Vigna unguiculata