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Literature summary extracted from

  • Nishimura, H.; Yoshida, K.; Yokota, Y.; Matsushima, A.; Inada, Y.
    Physicochemical properties and states of sulfhydryl groups of uricase from Candida utilis (1982), J. Biochem., 91, 41-48.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.3.3 dithiothreitol prevents polymerization and stabilizes throughout purification Cyberlindnera jadinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 5,5'-dithiobis(2-nitrobenzoate)
-
 Cyberlindnera jadinii
1.7.3.3 Cu2+
-
 Cyberlindnera jadinii
1.7.3.3 Hg2+
-
 Cyberlindnera jadinii
1.7.3.3 o-Iodosobenzoate
-
 Cyberlindnera jadinii
1.7.3.3 p-chloromercuribenzoate
-
 Cyberlindnera jadinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.01
-
 uric acid
-
 Cyberlindnera jadinii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.3.3 copper neither copper nor iron detected Cyberlindnera jadinii
1.7.3.3 Iron neither copper nor iron detected Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 30000
-
 alpha4, 4 * 30000, SDS-PAGE Cyberlindnera jadinii
1.7.3.3 120000
-
 gel filtration, equilibrium sedimentation Cyberlindnera jadinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Cyberlindnera jadinii
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Cyberlindnera jadinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Cyberlindnera jadinii 5-hydroxyisourate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.3.3 tetramer alpha4, 4 * 30000, SDS-PAGE Cyberlindnera jadinii