Literature summary extracted from

Kelly, D.P.; Wood, A.P.
Enzymes involved in microbiological oxidation of thiosulfate and polythionates (1994), Methods Enzymol., 243, 501-510.

No PubMed abstract available

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.8.2.2	periplasm	-	Thermithiobacillus tepidarius	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.8.2.2	20000	-	2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme	Acidiphilium acidophilum
1.8.2.2	24000	-	2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme	Acidiphilium acidophilum
1.8.2.2	45000	-	3 * 45000, SDS-PAGE	Thermithiobacillus tepidarius
1.8.2.2	102000	-	-	Acidiphilium acidophilum
1.8.2.2	138000	-	-	Thermithiobacillus tepidarius

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.8.2.2	Acidiphilium acidophilum	-	-	-
1.8.2.2	Halothiobacillus neapolitanus	-	-	-
1.8.2.2	Thermithiobacillus tepidarius	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.8.2.2	partial, using ion exchange chromatography, gel filtration and hydrophobic interaction chromatography	Thermithiobacillus tepidarius
1.8.2.2	using ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography and gel filtration	Acidiphilium acidophilum

Subunits

EC Number	Subunits	Comment	Organism
1.8.2.2	heterotetramer	2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme	Acidiphilium acidophilum
1.8.2.2	trimer	3 * 45000, SDS-PAGE	Thermithiobacillus tepidarius

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.8.2.2	4.5	-	-	Halothiobacillus neapolitanus
1.8.2.2	4.5	-	-	Thermithiobacillus tepidarius

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.8.2.2	heme c	5.3 mole per mol of native enzyme, made up of two different c-553 hemes, present in both subunit types	Acidiphilium acidophilum

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Release 2023.1 (January 2023)