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Literature summary extracted from

  • Tuttle, J.H.; Schwartz, J.H.; Whited, G.M.
    Some properties of thiosulfate-oxidizing enzyme from marine heterotroph 16B (1983), Appl. Environ. Microbiol., 46, 438-445.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.2 additional information
-
 additional information temperature dependency of Km Pseudomonas sp.
1.8.2.2 1.57
-
 thiosulfate pH 6.1-6.3, 30°C Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Pseudomonas sp.
-
 16B, marine heterotroph
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.2 partial, using ion-exchange chromatography and calcium phosphate gel treatment Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 11.38
-
 purified enzyme Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.8.2.2 -20°C, 36% loss of activity after 9 days Pseudomonas sp.
1.8.2.2 4°C, stable, 2 days Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 thiosulfate + oxidized acceptor only native cytochrome c acts as electron acceptor Pseudomonas sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide or native cytochrome c act as electron acceptors Pseudomonas sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Pseudomonas sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor the activity does not couple with mammalian cytochrome c as electron acceptor Pseudomonas sp. tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Pseudomonas sp. tetrathionate + reduced acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.2.2 30
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.2 100
-
 after 1 min the activity is completely destroyed Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 additional information
-
 no definite pH-optimum Pseudomonas sp.