EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.11 | overexpression of N-terminally His-tagged wild-type and mutant MsrAs in Escherichia coli | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.11 | C107S | site-directed mutagenesis, the mutant shows 14% increased activity with DTT and 4% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C107S/C218S | site-directed mutagenesis, the mutant shows 78% reduced activity with DTT and 94% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C107S/C218S/C227S | site-directed mutagenesis, the mutant shows 61% reduced activity with DTT and 92% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C107S/C227S | site-directed mutagenesis, the mutant shows 4% reduced activity with DTT and 86% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C218S | site-directed mutagenesis, the mutant shows 65% reduced activity with DTT and 78% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C218S/C227S | site-directed mutagenesis, the mutant shows 58% reduced activity with DTT and 96% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C227S | site-directed mutagenesis, the mutant shows 11% reduced activity with DTT and 81% with thioredoxin compared to the wild-type enzyme | Bos taurus |
1.8.4.11 | C72S | site-directed mutagenesis, inactive mutant, no disulfide bond in the mutant enzyme | Bos taurus |
1.8.4.11 | C72S/C107S/C227S | site-directed mutagenesis, inactuve mutant | Bos taurus |
1.8.4.11 | C72S/C218S | site-directed mutagenesis, inactive mutant | Bos taurus |
1.8.4.11 | additional information | free sulfhydryl content and disulfide bond numbers in wild-type and mutant enzymes, overview | Bos taurus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.11 | additional information | the enzyme does not require meal ions for activity, free sulfhydryl content and disulfide bond numbers in wild-type and mutant enzymes, overview | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.11 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.11 | recombinant N-terminally His-tagged wild-type and mutant MsrAs from Escherichia coli by nickel affinity chromatography and dialysis | Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.4.11 | peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin | catalytic mechanism, Cys72 is essential for activity forming disulfide bonds with either Cys218 or Cys227 | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.11 | N-acetyl-L-methionine-(S)-S-oxide + DTT | stereospecific reduction | Bos taurus | N-acetyl-L-methionine + DTT disulfide + H2O | - |
? | |
1.8.4.11 | N-acetyl-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction | Bos taurus | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.11 | MsrA | - |
Bos taurus |
1.8.4.11 | peptide methionine sulfoxide reductase | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 37 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 7.4 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.11 | dithiothreitol | - |
Bos taurus | |
1.8.4.11 | thioredoxin | - |
Bos taurus |