Identification and characterization of a putative active site for peptide methionine sulfoxide reductase (MsrA) and its substrate stereospecificity
Moskovitz, J.; Poston, J.M.; Berlett, B.S.; Nosworthy, N.J.; Szczepanowski, R.; Stadtman, E.R.; J. Biol. Chem. 275, 14167-14172 (2000) 
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.4.11
KCl
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
1.8.4.11
Na2SO4
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
1.8.4.11
NaCl
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
1.8.4.11
NaF
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.8.4.11
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
Saccharomyces cerevisiae
Engineering
EC Number
Protein Variants
Commentary
Organism
1.8.4.11
C25S
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
1.8.4.11
F26A
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
1.8.4.11
F26H
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
1.8.4.11
G24A
site-directed mutagenesis, 60% reduced activity compared to the wild-type enzyme
Saccharomyces cerevisiae
1.8.4.11
G28A
site-directed mutagenesis, 81% reduced activity compared to the wild-type enzyme
Saccharomyces cerevisiae
1.8.4.11
W27A
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.4.11
0.044
-
L-methionine (S)-sulfoxide
recombinant wild-type enzyme, pH 7.5, 37°C
Saccharomyces cerevisiae
1.8.4.11
0.12
-
L-methionine (S)-sulfoxide
recombinant wild-type enzyme, pH 7.5, 37°C
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.4.11
KCl
enzyme prefers high ionic strength, activation
Escherichia coli
1.8.4.11
Na2SO4
enzyme prefers high ionic strength, activation
Escherichia coli
1.8.4.11
NaCl
enzyme prefers high ionic strength, activation
Escherichia coli
1.8.4.11
NaF
enzyme prefers high ionic strength, activation
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
Escherichia coli
FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide
L-methionine + thioredoxin disulfide + H2O
-
-
?
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
Saccharomyces cerevisiae
MsrA is specific for the S-isomer
L-methionine + thioredoxin disulfide + H2O
-
-
?
1.8.4.12
L-methionine (R)-sulfoxide + thioredoxin
Homo sapiens
Msr is specific for the R-isomer
L-methionine + thioredoxin disulfide + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.8.4.11
Escherichia coli
-
-
-
1.8.4.11
Saccharomyces cerevisiae
-
-
-
1.8.4.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.8.4.11
partially
Escherichia coli
1.8.4.11
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
Saccharomyces cerevisiae
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.4.12
leukocyte
polymorphonuclear
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.11
additional information
-
-
Escherichia coli
1.8.4.11
additional information
-
-
Saccharomyces cerevisiae
1.8.4.11
4.2
-
purified recombinant wild-type enzyme, substrate L-methionine (S)-sulfoxide
Saccharomyces cerevisiae
1.8.4.11
43
-
purified recombinant wild-type enzyme, substrate dabsyl-L-methionine (S)-sulfoxide
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.8.4.11
(S)-methyl 4-tolyl sulfoxide + thioredoxin
-
394098
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.11
(S)-methyl 4-tolyl sulfoxide + thioredoxin
FMsr is specific for the S-isomer
394098
Escherichia coli
?
-
-
-
?
1.8.4.11
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
MsrA is specific for the S-isomer
394098
Saccharomyces cerevisiae
dabsyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
FMsr is specific for the S-isomer
394098
Escherichia coli
dabsyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide
394098
Escherichia coli
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
MsrA is specific for the S-isomer
394098
Saccharomyces cerevisiae
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
additional information
substrate specificity
394098
Escherichia coli
?
-
-
-
?
1.8.4.11
additional information
substrate specificity
394098
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.12
(R)-methyl 4-tolyl sulfoxide + thioredoxin
-
394098
Homo sapiens
?
-
-
-
?
1.8.4.12
dabsyl-L-methionine (R)-sulfoxide + thioredoxin
FMsr is specific for the R-isomer
394098
Homo sapiens
dabsyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.12
L-methionine (R)-sulfoxide + thioredoxin
Msr is specific for the R-isomer
394098
Homo sapiens
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.12
additional information
substrate specificity
394098
Homo sapiens
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.8.4.11
FMsr
-
Escherichia coli
1.8.4.11
methionine sulfoxide reductase
-
Escherichia coli
1.8.4.11
methionine sulfoxide reductase
-
Saccharomyces cerevisiae
1.8.4.11
MsrA
-
Saccharomyces cerevisiae
1.8.4.12
methionine sulfoxide reductase
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.11
37
-
assay at
Escherichia coli
1.8.4.11
37
-
assay at
Saccharomyces cerevisiae
1.8.4.12
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.11
7.5
-
assay at
Escherichia coli
1.8.4.11
7.5
-
assay at
Saccharomyces cerevisiae
1.8.4.12
7.5
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.4.11
thioredoxin
-
Escherichia coli
1.8.4.11
thioredoxin
-
Saccharomyces cerevisiae
1.8.4.12
thioredoxin
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.4.11
KCl
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
1.8.4.11
Na2SO4
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
1.8.4.11
NaCl
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
1.8.4.11
NaF
enzyme prefers high ionic strength, activation
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.4.11
expression of His-tagged wild-type and mutant enzymes in Escherichia coli
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.4.11
thioredoxin
-
Escherichia coli
1.8.4.11
thioredoxin
-
Saccharomyces cerevisiae
1.8.4.12
thioredoxin
-
Homo sapiens
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.8.4.11
C25S
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
1.8.4.11
F26A
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
1.8.4.11
F26H
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
1.8.4.11
G24A
site-directed mutagenesis, 60% reduced activity compared to the wild-type enzyme
Saccharomyces cerevisiae
1.8.4.11
G28A
site-directed mutagenesis, 81% reduced activity compared to the wild-type enzyme
Saccharomyces cerevisiae
1.8.4.11
W27A
site-directed mutagenesis, inactive mutant
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.4.11
0.044
-
L-methionine (S)-sulfoxide
recombinant wild-type enzyme, pH 7.5, 37°C
Saccharomyces cerevisiae
1.8.4.11
0.12
-
L-methionine (S)-sulfoxide
recombinant wild-type enzyme, pH 7.5, 37°C
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.4.11
KCl
enzyme prefers high ionic strength, activation
Escherichia coli
1.8.4.11
Na2SO4
enzyme prefers high ionic strength, activation
Escherichia coli
1.8.4.11
NaCl
enzyme prefers high ionic strength, activation
Escherichia coli
1.8.4.11
NaF
enzyme prefers high ionic strength, activation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
Escherichia coli
FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide
L-methionine + thioredoxin disulfide + H2O
-
-
?
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
Saccharomyces cerevisiae
MsrA is specific for the S-isomer
L-methionine + thioredoxin disulfide + H2O
-
-
?
1.8.4.12
L-methionine (R)-sulfoxide + thioredoxin
Homo sapiens
Msr is specific for the R-isomer
L-methionine + thioredoxin disulfide + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.4.11
partially
Escherichia coli
1.8.4.11
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli
Saccharomyces cerevisiae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.4.12
leukocyte
polymorphonuclear
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.4.11
additional information
-
-
Escherichia coli
1.8.4.11
additional information
-
-
Saccharomyces cerevisiae
1.8.4.11
4.2
-
purified recombinant wild-type enzyme, substrate L-methionine (S)-sulfoxide
Saccharomyces cerevisiae
1.8.4.11
43
-
purified recombinant wild-type enzyme, substrate dabsyl-L-methionine (S)-sulfoxide
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.8.4.11
(S)-methyl 4-tolyl sulfoxide + thioredoxin
-
394098
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.11
(S)-methyl 4-tolyl sulfoxide + thioredoxin
FMsr is specific for the S-isomer
394098
Escherichia coli
?
-
-
-
?
1.8.4.11
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
MsrA is specific for the S-isomer
394098
Saccharomyces cerevisiae
dabsyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
dabsyl-L-methionine (S)-sulfoxide + thioredoxin
FMsr is specific for the S-isomer
394098
Escherichia coli
dabsyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide
394098
Escherichia coli
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
L-methionine (S)-sulfoxide + thioredoxin
MsrA is specific for the S-isomer
394098
Saccharomyces cerevisiae
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.11
additional information
substrate specificity
394098
Escherichia coli
?
-
-
-
?
1.8.4.11
additional information
substrate specificity
394098
Saccharomyces cerevisiae
?
-
-
-
?
1.8.4.12
(R)-methyl 4-tolyl sulfoxide + thioredoxin
-
394098
Homo sapiens
?
-
-
-
?
1.8.4.12
dabsyl-L-methionine (R)-sulfoxide + thioredoxin
FMsr is specific for the R-isomer
394098
Homo sapiens
dabsyl-L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.12
L-methionine (R)-sulfoxide + thioredoxin
Msr is specific for the R-isomer
394098
Homo sapiens
L-methionine + thioredoxin disulfide + H2O
-
-
-
?
1.8.4.12
additional information
substrate specificity
394098
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.4.11
37
-
assay at
Escherichia coli
1.8.4.11
37
-
assay at
Saccharomyces cerevisiae
1.8.4.12
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.4.11
7.5
-
assay at
Escherichia coli
1.8.4.11
7.5
-
assay at
Saccharomyces cerevisiae
1.8.4.12
7.5
-
assay at
Homo sapiens