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Literature summary extracted from
- Identification and characterization of a putative active site for peptide methionine sulfoxide reductase (MsrA) and its substrate stereospecificity (2000), J. Biol. Chem., 275, 14167-14172.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | KCl | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae |  |
| 1.8.4.11 | Na2SO4 | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
| 1.8.4.11 | NaCl | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
| 1.8.4.11 | NaF | enzyme prefers high ionic strength, activation | Saccharomyces cerevisiae | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | C25S | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| 1.8.4.11 | F26A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| 1.8.4.11 | F26H | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
| 1.8.4.11 | G24A | site-directed mutagenesis, 60% reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
| 1.8.4.11 | G28A | site-directed mutagenesis, 81% reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
| 1.8.4.11 | W27A | site-directed mutagenesis, inactive mutant | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.11 | 0.044 | - |
L-methionine (S)-sulfoxide | recombinant wild-type enzyme, pH 7.5, 37°C | Saccharomyces cerevisiae | |
| 1.8.4.11 | 0.12 | - |
L-methionine (S)-sulfoxide | recombinant wild-type enzyme, pH 7.5, 37°C | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | KCl | enzyme prefers high ionic strength, activation | Escherichia coli | |
| 1.8.4.11 | Na2SO4 | enzyme prefers high ionic strength, activation | Escherichia coli | |
| 1.8.4.11 | NaCl | enzyme prefers high ionic strength, activation | Escherichia coli | |
| 1.8.4.11 | NaF | enzyme prefers high ionic strength, activation | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Saccharomyces cerevisiae | MsrA is specific for the S-isomer | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Homo sapiens | Msr is specific for the R-isomer | L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Escherichia coli | - |
- |
- |
| 1.8.4.11 | Saccharomyces cerevisiae | - |
- |
- |
| 1.8.4.12 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | partially | Escherichia coli |
| 1.8.4.11 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.4.12 | leukocyte | polymorphonuclear | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | additional information | - |
- |
Escherichia coli |
| 1.8.4.11 | additional information | - |
- |
Saccharomyces cerevisiae |
| 1.8.4.11 | 4.2 | - |
purified recombinant wild-type enzyme, substrate L-methionine (S)-sulfoxide | Saccharomyces cerevisiae |
| 1.8.4.11 | 43 | - |
purified recombinant wild-type enzyme, substrate dabsyl-L-methionine (S)-sulfoxide | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | (S)-methyl 4-tolyl sulfoxide + thioredoxin | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 1.8.4.11 | (S)-methyl 4-tolyl sulfoxide + thioredoxin | FMsr is specific for the S-isomer | Escherichia coli | ? | - |
? | |
| 1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-isomer | Saccharomyces cerevisiae | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + thioredoxin | FMsr is specific for the S-isomer | Escherichia coli | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | FMsr is absolutely specific for the S-isomer of free methionine sulfoxide, no activity with protein bound methionine sulfoxide | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-isomer | Saccharomyces cerevisiae | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | substrate specificity | Escherichia coli | ? | - |
? | |
| 1.8.4.11 | additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
| 1.8.4.12 | (R)-methyl 4-tolyl sulfoxide + thioredoxin | - |
Homo sapiens | ? | - |
? | |
| 1.8.4.12 | dabsyl-L-methionine (R)-sulfoxide + thioredoxin | FMsr is specific for the R-isomer | Homo sapiens | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Msr is specific for the R-isomer | Homo sapiens | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | substrate specificity | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | FMsr | - |
Escherichia coli |
| 1.8.4.11 | methionine sulfoxide reductase | - |
Escherichia coli |
| 1.8.4.11 | methionine sulfoxide reductase | - |
Saccharomyces cerevisiae |
| 1.8.4.11 | MsrA | - |
Saccharomyces cerevisiae |
| 1.8.4.12 | methionine sulfoxide reductase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 37 | - |
assay at | Escherichia coli |
| 1.8.4.11 | 37 | - |
assay at | Saccharomyces cerevisiae |
| 1.8.4.12 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.5 | - |
assay at | Escherichia coli |
| 1.8.4.11 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
| 1.8.4.12 | 7.5 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | thioredoxin | - |
Escherichia coli | |
| 1.8.4.11 | thioredoxin | - |
Saccharomyces cerevisiae | |
| 1.8.4.12 | thioredoxin | - |
Homo sapiens | |
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