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Literature summary extracted from

  • Nakamura, K.; Yoshikawa, H.; Okubo, S.; Kurosawa, H.; Amano, Y.
    Purification and properties of membrane-bound sulfite dehydrogenase from Thiobacillus thiooxidans JCM7814 (1995), Biosci. Biotechnol. Biochem., 59, 11-15.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.2.1 Triton X-100 increases activity Acidithiobacillus thiooxidans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.1 arsenite 1 mM, complete inhibition Acidithiobacillus thiooxidans
1.8.2.1 HgCl2 1 mM, 91% inhibition Acidithiobacillus thiooxidans
1.8.2.1 monoiodoacetate 1 mM, 19% inhibition Acidithiobacillus thiooxidans
1.8.2.1 NEM 1 mM, 53% inhibition Acidithiobacillus thiooxidans
1.8.2.1 PCMB 1 mM, 47% loss of activity Acidithiobacillus thiooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 1.95
-
 sulfite
-
 Acidithiobacillus thiooxidans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.2.1 membrane bound to Acidithiobacillus thiooxidans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.1 62000
-
 x * 62000 + x * 70000 + x * 74000, SDS-PAGE Acidithiobacillus thiooxidans
1.8.2.1 70000
-
 x * 62000 + x * 70000 + x * 74000, SDS-PAGE Acidithiobacillus thiooxidans
1.8.2.1 74000
-
 x * 62000 + x * 70000 + x * 74000, SDS-PAGE Acidithiobacillus thiooxidans
1.8.2.1 400000
-
 gel filtration Acidithiobacillus thiooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Acidithiobacillus thiooxidans
-
 JCM7814
-
1.8.2.1 Acidithiobacillus thiooxidans JCM 7814
-
 JCM7814
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1
-
 Acidithiobacillus thiooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.1 21
-
-
 Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricyanide + H2O
-
 Acidithiobacillus thiooxidans sulfate + ferrocyanide + H+
-
 ?
1.8.2.1 sulfite + ferricyanide + H2O
-
 Acidithiobacillus thiooxidans JCM 7814 sulfate + ferrocyanide + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O
-
 Acidithiobacillus thiooxidans sulfate + ferrocytochrome c + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O
-
 Acidithiobacillus thiooxidans JCM 7814 sulfate + ferrocytochrome c + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.1 ? x * 62000 + x * 70000 + x * 74000, SDS-PAGE Acidithiobacillus thiooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.2.1 20
-
-
 Acidithiobacillus thiooxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.1 30
-
 1 h, stable below Acidithiobacillus thiooxidans
1.8.2.1 40
-
 1 h, about 20% loss of activity Acidithiobacillus thiooxidans
1.8.2.1 50
-
 1 h, complete loss of activity Acidithiobacillus thiooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.1 7.5
-
-
 Acidithiobacillus thiooxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.2.1 7
-
 20°C, 1 h, stable Acidithiobacillus thiooxidans
1.8.2.1 9
-
 20°C, 1 h, about 20% loss of activity Acidithiobacillus thiooxidans