BRENDA - Enzyme Database show

Characterization of a new type of sulfite dehydrogenase from Paracoccus pantotrophus GB17

Quentmeier, A.; Kraft, R.; Kostka, S.; Klockenkamper, R.; Friedrich, C.G.; Arch. Microbiol. 173, 117-125 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.2.1
Ni2+
1 mM, 32% inhibition
Paracoccus pantotrophus
1.8.2.1
phosphate
-
Paracoccus pantotrophus
1.8.2.1
SO42-
-
Paracoccus pantotrophus
1.8.2.1
Zn2+
1 mM, 27% inhibition
Paracoccus pantotrophus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
0.007
-
sulfite
-
Paracoccus pantotrophus
1.8.2.1
0.019
-
cytochrome c
-
Paracoccus pantotrophus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.2.1
periplasm
-
Paracoccus pantotrophus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.2.1
Iron
enzyme contains 3.17 mol of iron per mol of enzyme
Paracoccus pantotrophus
1.8.2.1
Mg2+
1 mM, 150% increase in activity
Paracoccus pantotrophus
1.8.2.1
Mn2+
1 mM, 127% increase in activity
Paracoccus pantotrophus
1.8.2.1
Molybdenum
the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme
Paracoccus pantotrophus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
38815
-
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
43897
-
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
47000
-
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
50000
-
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
190000
-
native gradient PAGE
Paracoccus pantotrophus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.2.1
Paracoccus pantotrophus
-
GB17
-
1.8.2.1
Paracoccus pantotrophus GB17
-
GB17
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.2.1
-
Paracoccus pantotrophus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8.2.1
0.049
-
-
Paracoccus pantotrophus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394084
Paracoccus pantotrophus
sulfate + ferrocytochrome c + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394084
Paracoccus pantotrophus GB17
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.2.1
tetramer
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry; alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.2.1
6.5
-
-
Paracoccus pantotrophus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.2.1
heme
the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme
Paracoccus pantotrophus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.2.1
45
-
phosphate
-
Paracoccus pantotrophus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.2.1
heme
the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme
Paracoccus pantotrophus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.2.1
Ni2+
1 mM, 32% inhibition
Paracoccus pantotrophus
1.8.2.1
phosphate
-
Paracoccus pantotrophus
1.8.2.1
SO42-
-
Paracoccus pantotrophus
1.8.2.1
Zn2+
1 mM, 27% inhibition
Paracoccus pantotrophus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.2.1
45
-
phosphate
-
Paracoccus pantotrophus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
0.007
-
sulfite
-
Paracoccus pantotrophus
1.8.2.1
0.019
-
cytochrome c
-
Paracoccus pantotrophus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.2.1
periplasm
-
Paracoccus pantotrophus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.2.1
Iron
enzyme contains 3.17 mol of iron per mol of enzyme
Paracoccus pantotrophus
1.8.2.1
Mg2+
1 mM, 150% increase in activity
Paracoccus pantotrophus
1.8.2.1
Mn2+
1 mM, 127% increase in activity
Paracoccus pantotrophus
1.8.2.1
Molybdenum
the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme
Paracoccus pantotrophus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
38815
-
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
43897
-
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
47000
-
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
50000
-
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
190000
-
native gradient PAGE
Paracoccus pantotrophus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.2.1
-
Paracoccus pantotrophus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8.2.1
0.049
-
-
Paracoccus pantotrophus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394084
Paracoccus pantotrophus
sulfate + ferrocytochrome c + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394084
Paracoccus pantotrophus GB17
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.2.1
tetramer
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry; alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.2.1
6.5
-
-
Paracoccus pantotrophus