Data extracted from this reference:
Inhibitors
1.8.2.1
Ni2+
1 mM, 32% inhibition
Paracoccus pantotrophus
1.8.2.1
phosphate
Paracoccus pantotrophus
1.8.2.1
SO42-
Paracoccus pantotrophus
1.8.2.1
Zn2+
1 mM, 27% inhibition
Paracoccus pantotrophus
KM Value [mM]
1.8.2.1
0.007
sulfite
Paracoccus pantotrophus
1.8.2.1
0.019
cytochrome c
Paracoccus pantotrophus
Localization
1.8.2.1
periplasm
Paracoccus pantotrophus
Metals/Ions
1.8.2.1
Iron
enzyme contains 3.17 mol of iron per mol of enzyme
Paracoccus pantotrophus
1.8.2.1
Mg2+
1 mM, 150% increase in activity
Paracoccus pantotrophus
1.8.2.1
Mn2+
1 mM, 127% increase in activity
Paracoccus pantotrophus
1.8.2.1
Molybdenum
the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme
Paracoccus pantotrophus
Molecular Weight [Da]
1.8.2.1
38815
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
43897
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
47000
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
50000
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
190000
native gradient PAGE
Paracoccus pantotrophus
Organism
1.8.2.1
Paracoccus pantotrophus
GB17
1.8.2.1
Paracoccus pantotrophus GB17
GB17
Purification (Commentary)
1.8.2.1
Paracoccus pantotrophus
Specific Activity [micromol/min/mg]
1.8.2.1
0.049
Paracoccus pantotrophus
Substrates and Products (Substrate)
1.8.2.1
sulfite + ferricytochrome c + H2O
394084
Paracoccus pantotrophus
sulfate + ferrocytochrome c + H+
?
1.8.2.1
sulfite + ferricytochrome c + H2O
394084
Paracoccus pantotrophus GB17
sulfate + ferrocytochrome c + H+
?
Subunits
1.8.2.1
tetramer
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry; alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
pH Optimum
1.8.2.1
6.5
Paracoccus pantotrophus
Cofactor
1.8.2.1
heme
the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme
Paracoccus pantotrophus
Ki Value [mM]
1.8.2.1
45
phosphate
Paracoccus pantotrophus
Cofactor (protein specific)
1.8.2.1
heme
the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme
Paracoccus pantotrophus
Inhibitors (protein specific)
1.8.2.1
Ni2+
1 mM, 32% inhibition
Paracoccus pantotrophus
1.8.2.1
phosphate
Paracoccus pantotrophus
1.8.2.1
SO42-
Paracoccus pantotrophus
1.8.2.1
Zn2+
1 mM, 27% inhibition
Paracoccus pantotrophus
Ki Value [mM] (protein specific)
1.8.2.1
45
phosphate
Paracoccus pantotrophus
KM Value [mM] (protein specific)
1.8.2.1
0.007
sulfite
Paracoccus pantotrophus
1.8.2.1
0.019
cytochrome c
Paracoccus pantotrophus
Localization (protein specific)
1.8.2.1
periplasm
Paracoccus pantotrophus
Metals/Ions (protein specific)
1.8.2.1
Iron
enzyme contains 3.17 mol of iron per mol of enzyme
Paracoccus pantotrophus
1.8.2.1
Mg2+
1 mM, 150% increase in activity
Paracoccus pantotrophus
1.8.2.1
Mn2+
1 mM, 127% increase in activity
Paracoccus pantotrophus
1.8.2.1
Molybdenum
the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme
Paracoccus pantotrophus
Molecular Weight [Da] (protein specific)
1.8.2.1
38815
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
43897
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry
Paracoccus pantotrophus
1.8.2.1
47000
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
50000
alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
1.8.2.1
190000
native gradient PAGE
Paracoccus pantotrophus
Purification (Commentary) (protein specific)
1.8.2.1
Paracoccus pantotrophus
Specific Activity [micromol/min/mg] (protein specific)
1.8.2.1
0.049
Paracoccus pantotrophus
Substrates and Products (Substrate) (protein specific)
1.8.2.1
sulfite + ferricytochrome c + H2O
394084
Paracoccus pantotrophus
sulfate + ferrocytochrome c + H+
?
1.8.2.1
sulfite + ferricytochrome c + H2O
394084
Paracoccus pantotrophus GB17
sulfate + ferrocytochrome c + H+
?
Subunits (protein specific)
1.8.2.1
tetramer
2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry; alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE
Paracoccus pantotrophus
pH Optimum (protein specific)
1.8.2.1
6.5
Paracoccus pantotrophus