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Literature summary extracted from
- Purification of Thiobacillus novellus sulfite oxidase. Evidence for the presence of heme and molybdenum (1983), J. Biol. Chem., 258, 6762-6766.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.8.2.1 | proteolytic treatment with trypsin at room temperature for up to 5 h at trypsin to enzyme ratios of 1/20 and 1/1 does not affect the activity of the enzyme | Starkeya novella |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.2.1 | Molybdenum | enzyme contains molybdenum | Starkeya novella |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.1 | 40000 | - |
gel filtration | Starkeya novella |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.2.1 | Starkeya novella | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.2.1 | - |
Starkeya novella |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.1 | additional information | - |
- |
Starkeya novella |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.2.1 | sulfite + ferricyanide + H2O | 3-5% of the activity with ferricytochrome | Starkeya novella | sulfate + ferrocyanide + H+ | - |
? | |
| 1.8.2.1 | sulfite + ferricytochrome c + H2O | - |
Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.2.1 | monomer | 1 * 40000, SDS-PAGE | Starkeya novella |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.1 | 58 | - |
rapid inactivation of oxidized enzyme, substrate-reduced enzyme remains active for more than 1 h | Starkeya novella |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.2.1 | 8.5 | - |
- |
Starkeya novella |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.2.1 | heme | enzyme contains heme | Starkeya novella | |
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Release 2023.1 (January 2023)
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