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Literature summary extracted from

  • Kato, Y.; Urano, J.i.; Maki, Y.; Ushimaru, T.
    Purification and characterization of dehydroascorbate reductase from rice (1997), Plant Cell Physiol., 38, 173-178.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.5.1 2-mercaptoethanol 5 mM, enhances the activity by 13% Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.5.1 iodoacetic acid 0.1 mM, 48% loss of activity. 1.0 mM, 91% loss of activity Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.1 0.35
-
 dehydroascorbate
-
 Oryza sativa
1.8.5.1 0.84
-
 GSH
-
 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.5.1 26000
-
 1 * 26000, SDS-PAGE Oryza sativa
1.8.5.1 27000
-
 gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate Oryza sativa the enzyme is critical for maintenance of an appropriate level of ascorbate in plant cells GSSG + ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.1 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.1
-
 Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.5.1 49.1
-
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.1 glutathione + dehydroascorbate the enzyme is critical for maintenance of an appropriate level of ascorbate in plant cells Oryza sativa GSSG + ascorbate
-
 ?
1.8.5.1 GSH + dehydroascorbate
-
 Oryza sativa GSSG + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.5.1 monomer 1 * 26000, SDS-PAGE Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.5.1 70
-
 10 min, 12% loss of activity Oryza sativa
1.8.5.1 80
-
 10 min, 10% loss of activity Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.5.1 8.2
-
-
 Oryza sativa