EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.2.6 | overexpression in Escherichia coli BL21 | Glycine max |
1.8.1.4 | overexpression in Escherichia coli | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.6 | 0.029 | - |
ferrileghemoglobin | recombinant FLbR-2 | Glycine max | |
1.6.2.6 | 0.058 | - |
NADH | recombinant FLbR-2 | Glycine max | |
1.6.2.6 | 3.381 | - |
Lipoamide | recombinant FLbR-2 | Glycine max | |
1.8.1.4 | 0.029 | - |
ferrileghemoglobin | - |
Glycine max | |
1.8.1.4 | 0.058 | - |
NADH | - |
Glycine max | |
1.8.1.4 | 3.38 | - |
Lipoamide | - |
Glycine max |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.2.6 | mitochondrion | - |
Glycine max | 5739 | - |
1.8.1.4 | mitochondrion | - |
Glycine max | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.2.6 | 50030 | - |
electrospray mass spectrometry | Glycine max |
1.8.1.4 | 50027 | - |
x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis | Glycine max |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.6 | Glycine max | O81413 | - |
- |
1.8.1.4 | Glycine max | O81413 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.6 | - |
Glycine max |
1.8.1.4 | - |
Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.2.6 | leaf | - |
Glycine max | - |
1.6.2.6 | root | - |
Glycine max | - |
1.6.2.6 | root nodule | - |
Glycine max | - |
1.6.2.6 | stem | - |
Glycine max | - |
1.8.1.4 | nodule | enzyme form FlbR-2 | Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 83.06 | - |
lipoamide reduction | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.6 | NADH + ferrileghemoglobin | - |
Glycine max | NAD+ + ferroleghemoglobin | - |
? | |
1.6.2.6 | NADH + lipoamide | - |
Glycine max | NAD+ + ? | - |
? | |
1.8.1.4 | dihydrolipoamide + NAD+ | - |
Glycine max | lipoamide + NADH | - |
? | |
1.8.1.4 | ferrileghemoglobin + NADH + H+ | - |
Glycine max | ferroleghemoglobin + NAD+ | - |
r | |
1.8.1.4 | lipoamide + NADH | - |
Glycine max | dihydrolipoamide + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.4 | dimer | x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis | Glycine max |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.6.2.6 | FLbR-2 | - |
Glycine max |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.6 | 1.6 | - |
ferrileghemoglobin | recombinant FLbR-2 | Glycine max | |
1.6.2.6 | 137 | - |
NADH | recombinant FLbR-2 | Glycine max | |
1.6.2.6 | 392 | - |
Lipoamide | recombinant FLbR-2 | Glycine max |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.6 | FAD | not covalently bound to the protein | Glycine max | |
1.8.1.4 | FAD | covalently bound to the protein | Glycine max | |
1.8.1.4 | NAD+ | - |
Glycine max | |
1.8.1.4 | NADH | - |
Glycine max |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.6.2.6 | Glycine max | isoelectric focusing, pH-range 4.0-6.5 | - |
5.8 |