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Literature summary extracted from

  • Komuniecki, R.; Saz, H.J.
    Purification of lipoamide dehydrogenase from Ascaris muscle mitochondria and its relationship to NADH:NAD+ transhydrogenase activity (1979), Arch. Biochem. Biophys., 196, 239-247.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 NADH competitive with respect to NAD+ Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.0085
-
 NADH NADH:2,6-dichlorophenol indophenol reductase activity Ascaris suum
1.8.1.4 0.009
-
 NADH NADH:NAD+ transhydrogenase activity Ascaris suum
1.8.1.4 0.01
-
 NADH lipoamide dehydrogenase activity Ascaris suum
1.8.1.4 0.037
-
 NAD+
-
 Ascaris suum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.4 mitochondrion
-
 Ascaris suum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 54000
-
 2 * 54000, SDS-PAGE Ascaris suum
1.8.1.4 105000
-
 gel filtration Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Ascaris suum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4
-
 Ascaris suum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 muscle
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 90.2
-
-
 Ascaris suum

Storage Stability

EC Number Storage Stability Organism
1.8.1.4 -20°C, stable for at least 6 months Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Ascaris suum lipoamide + NADH
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Ascaris suum lipoamide + NADH
-
 r
1.8.1.4 additional information NADH:NAD+ transhydrogenase activity Ascaris suum ?
-
 ?
1.8.1.4 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Ascaris suum ? + NAD+
-
 ?
1.8.1.4 thio-NAD+ + NADH
-
 Ascaris suum thio-NADH + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 dimer 2 * 54000, SDS-PAGE Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 6.4
-
 reduction of lipoamide Ascaris suum
1.8.1.4 7.4
-
 reduction of 2,6-dichlorophenol indophenol Ascaris suum
1.8.1.4 7.5
-
 reduction of thio-NAD+ Ascaris suum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD contains 1 FAD per subunit Ascaris suum
1.8.1.4 NAD+
-
 Ascaris suum
1.8.1.4 NADH
-
 Ascaris suum