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Literature summary extracted from
- Dihydrolipoamide dehydrogenase component of the pyruvate dehydrogenase complex from Escherichia coli K12. Comparative characterization of the free and the complex-bound component (1981), Eur. J. Biochem., 114, 413-420.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | NADH | - |
Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.4 | 0.08 | - |
dihydrolipoamide | complex-bound dihydrolipoamide dehydrogenase | Escherichia coli | |
| 1.8.1.4 | 0.28 | - |
dihydrolipoamide | free dihydrolipoamide dehydrogenase | Escherichia coli | |
| 1.8.1.4 | 0.4 | - |
NAD+ | complex-bound dihydrolipoamide dehydrogenase | Escherichia coli | |
| 1.8.1.4 | 1.83 | - |
NAD+ | free dihydrolipoamide dehydrogenase | Escherichia coli | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 56000 | - |
2 * 56000, SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.4 | Escherichia coli | - |
K-12 | - |
| 1.8.1.4 | Escherichia coli | - |
diyhdrolipoamide dehydrogenase component of the pyruvate dehydrogenase complex | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.4 | from pyruvate dehydrogenase complex | Escherichia coli |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.4 | protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping-pong mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.4 | dihydrolipoamide + NAD+ | - |
Escherichia coli | lipoamide + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.4 | dimer | 2 * 56000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.4 | additional information | - |
additional information | - |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 8 | - |
- |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.4 | 6.5 | 8.5 | pH 6.5: about 40% of maximal activity, pH 8.5: about 50% of maximal activity | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.4 | FAD | - |
Escherichia coli | |
| 1.8.1.4 | NAD+ | - |
Escherichia coli | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.4 | 0.009 | - |
NADH | free dihydrolipoamide dehydrogenase | Escherichia coli | |
| 1.8.1.4 | 0.016 | - |
NADH | complex-bound dihydrolipoamide dehydrogenase | Escherichia coli | |
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Release 2023.1 (January 2023)
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