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Literature summary extracted from

  • Heinrich, P.; Ronft, H.; Schartau, W.; Kresze, G.B.
    Lipoamide dehydrogenase from bakers yeast. Improved purification and some molecular, kinetic, and immunochemical properties (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 41-50.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.4 trypsin or papain, treatment with 0.019 mg/ml protease Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 NADH competitive with respect to NAD+ Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.15
-
 NAD+
-
 Saccharomyces cerevisiae
1.8.1.4 0.48
-
 dihydrolipoamide
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 56000
-
 2 * 56000, SDS-PAGE Saccharomyces cerevisiae
1.8.1.4 117000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 843
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Saccharomyces cerevisiae lipoamide + NADH
-
 ?
1.8.1.4 hexacyanoferrate + NADH
-
 Saccharomyces cerevisiae ? + NAD+
-
 ?
1.8.1.4 iodonitrotetrazolium + NADH
-
 Saccharomyces cerevisiae ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 dimer 2 * 56000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 8.5
-
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD contains 1 FAD per subunit Saccharomyces cerevisiae
1.8.1.4 NAD+
-
 Saccharomyces cerevisiae
1.8.1.4 NADH
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.4 0.031
-
 NADH
-
 Saccharomyces cerevisiae