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Literature summary extracted from

  • Dietrichs, D.; Andreesen, J.R.
    Purification and comparative studies of dihydrolipoamide dehydrogenases from the anaerobic, glycine-utilizing bacteria Peptostreptococcus glycinophilus, Clostridium cylindrosporum, and Clostridium sporogenes (1990), J. Bacteriol., 172, 243-251.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.047
-
 NADH
-
 Peptostreptococcus glycinophilus
1.8.1.4 0.2
-
 NAD+
-
 Clostridium cylindrosporum
1.8.1.4 0.25
-
 NAD+
-
 Peptostreptococcus glycinophilus
1.8.1.4 0.27
-
 NADH
-
 Clostridium cylindrosporum
1.8.1.4 0.5
-
 NAD+
-
 Clostridium cylindrosporum
1.8.1.4 0.83
-
 NADP+
-
 Clostridium cylindrosporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 52000
-
 2 * 52000, SDS-PAGE Clostridium cylindrosporum
1.8.1.4 53000
-
 2 * 53000, SDS-PAGE Peptostreptococcus glycinophilus
1.8.1.4 105000
-
 gel filtration Clostridium cylindrosporum
1.8.1.4 105000
-
 gel filtration Peptostreptococcus glycinophilus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Clostridium cylindrosporum
-
-
-
1.8.1.4 Peptostreptococcus glycinophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4
-
 Clostridium cylindrosporum
1.8.1.4
-
 Peptostreptococcus glycinophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 214
-
-
 Peptostreptococcus glycinophilus
1.8.1.4 483
-
-
 Clostridium cylindrosporum

Storage Stability

EC Number Storage Stability Organism
1.8.1.4 -20°C, 6 months, about 10% loss of activity Peptostreptococcus glycinophilus
1.8.1.4 -20°C, 6 months, about 60% loss of activity Clostridium cylindrosporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Clostridium cylindrosporum lipoamide + NADH
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Peptostreptococcus glycinophilus lipoamide + NADH
-
 ?
1.8.1.4 dihydrolipoamide + NADP+
-
 Clostridium cylindrosporum lipoamide + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.4 dimer 2 * 53000, SDS-PAGE Peptostreptococcus glycinophilus
1.8.1.4 dimer 2 * 52000, SDS-PAGE Clostridium cylindrosporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 7
-
 reaction with NAD(P)H and lipoamide Clostridium cylindrosporum
1.8.1.4 7
-
 reaction with NAD(P)H and lipoamide Peptostreptococcus glycinophilus
1.8.1.4 9
-
 reaction with NAD(P)+ and dihydrolipoamide Clostridium cylindrosporum
1.8.1.4 9
-
 reaction with NAD(P)+ and dihydrolipoamide Peptostreptococcus glycinophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD contains 1 FAD per subunit Clostridium cylindrosporum
1.8.1.4 FAD contains 1 FAD per subunit Peptostreptococcus glycinophilus
1.8.1.4 NAD+
-
 Clostridium cylindrosporum
1.8.1.4 NAD+ highly specific for Peptostreptococcus glycinophilus
1.8.1.4 NADP+
-
 Clostridium cylindrosporum
1.8.1.4 NADP+ no activity Peptostreptococcus glycinophilus
1.8.1.4 NADPH
-
 Clostridium cylindrosporum
1.8.1.4 NADPH no activity Peptostreptococcus glycinophilus