BRENDA - Enzyme Database show

Sulfite reductase and APS reductase from Archaeoglobus fulgidus

Dahl, C.; Truper, H.G.; Methods Enzymol. 331, 427-441 (2001)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.99.2
Iron
contains 8 mol of nonheme iron per mol of enzyme
Archaeoglobus fulgidus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.99.2
17100
-
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
Archaeoglobus fulgidus
1.8.99.2
73300
-
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
Archaeoglobus fulgidus
1.8.99.2
160000
-
gel filtration
Archaeoglobus fulgidus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.99.2
Archaeoglobus fulgidus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.99.2
-
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.99.2
additional information
-
-
Archaeoglobus fulgidus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.99.2
ADP + sulfite + ferricyanide
50% of the activity with AMP
393954
Archaeoglobus fulgidus
? + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
no activity with cytochrome c
393954
Archaeoglobus fulgidus
adenylyl sulfate + ?
-
-
-
-
1.8.99.2
AMP + sulfite + ferricyanide
-
393954
Archaeoglobus fulgidus
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
ATP + sulfite + ferricyanide
24% of the activity with AMP
393954
Archaeoglobus fulgidus
? + ferrocyanide
-
-
-
?
1.8.99.2
deoxyAMP + sulfite + ferricyanide
74% of the activity with AMP
393954
Archaeoglobus fulgidus
deoxyadenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
GMP + sulfite + ferricyanide
68% of the activity with AMP
393954
Archaeoglobus fulgidus
guanylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
IMP + sulfite + ferricyanide
74% of the activity with AMP
393954
Archaeoglobus fulgidus
? + ferrocyanide
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.99.2
tetramer
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
Archaeoglobus fulgidus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme; noncovalently bound
Archaeoglobus fulgidus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme; noncovalently bound
Archaeoglobus fulgidus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.99.2
Iron
contains 8 mol of nonheme iron per mol of enzyme
Archaeoglobus fulgidus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.99.2
17100
-
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
Archaeoglobus fulgidus
1.8.99.2
73300
-
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
Archaeoglobus fulgidus
1.8.99.2
160000
-
gel filtration
Archaeoglobus fulgidus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.99.2
-
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.99.2
additional information
-
-
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.99.2
ADP + sulfite + ferricyanide
50% of the activity with AMP
393954
Archaeoglobus fulgidus
? + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
no activity with cytochrome c
393954
Archaeoglobus fulgidus
adenylyl sulfate + ?
-
-
-
-
1.8.99.2
AMP + sulfite + ferricyanide
-
393954
Archaeoglobus fulgidus
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
ATP + sulfite + ferricyanide
24% of the activity with AMP
393954
Archaeoglobus fulgidus
? + ferrocyanide
-
-
-
?
1.8.99.2
deoxyAMP + sulfite + ferricyanide
74% of the activity with AMP
393954
Archaeoglobus fulgidus
deoxyadenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
GMP + sulfite + ferricyanide
68% of the activity with AMP
393954
Archaeoglobus fulgidus
guanylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
IMP + sulfite + ferricyanide
74% of the activity with AMP
393954
Archaeoglobus fulgidus
? + ferrocyanide
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.99.2
tetramer
alpha2beta2, 2 * 73300 + 2 * 17100, SDS-PAGE
Archaeoglobus fulgidus