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Literature summary extracted from

  • Trueper, H.G.; Rogers, L.A.
    Purification and properties of adenylyl sulfate reductase from phototrophic sulfur bacterium, Thiocapsa roseopersicina (1971), J. Bacteriol., 108, 1112-1121.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.99.2 iodoacetamide 10 mM 2% loss of the activity with ferricyanide, 19% loss of the activity with cytochrome c Thiocapsa roseopersicina
1.8.99.2 NaN3 10 mM, no loss of the activity with ferricyanide, 52% loss of the activity with cytochrome c Thiocapsa roseopersicina
1.8.99.2 NEM 10 mM, 45% loss of activity with ferricyanide, complete loss of activity with cytochrome c Thiocapsa roseopersicina
1.8.99.2 PCMB 1 mM, 23% loss of the activity with ferricyanide, complete loss of activity with cytochrome c Thiocapsa roseopersicina
1.8.99.2 sodium arsenite 10 mM, no loss of the activity with ferricyanide, 88% loss of the activity with cytochrome c Thiocapsa roseopersicina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.99.2 0.033
-
 cytochrome c
-
 Thiocapsa roseopersicina
1.8.99.2 0.05
-
 AMP reaction with cytochrome c Thiocapsa roseopersicina
1.8.99.2 0.073
-
 sulfite reaction with ferricyanide Thiocapsa roseopersicina
1.8.99.2 0.093
-
 sulfite reaction with cytochrome c Thiocapsa roseopersicina
1.8.99.2 0.13
-
 ferricyanide
-
 Thiocapsa roseopersicina
1.8.99.2 1.5
-
 sulfite reaction with ferricyanide Thiocapsa roseopersicina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.99.2 Iron contains 4 mol of non-heme iron per mol of enzyme Thiocapsa roseopersicina

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Thiocapsa roseopersicina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2
-
 Thiocapsa roseopersicina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.99.2 additional information
-
-
 Thiocapsa roseopersicina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 AMP + sulfite + cytochrome c
-
 Thiocapsa roseopersicina adenylyl sulfate + ?
-
 ?
1.8.99.2 AMP + sulfite + ferricyanide
-
 Thiocapsa roseopersicina adenylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 CMP + sulfite + cytochrome c 7% of the activity with AMP Thiocapsa roseopersicina cytidylyl sulfate + ?
-
 ?
1.8.99.2 CMP + sulfite + ferricyanide 8% of the activity with AMP Thiocapsa roseopersicina cytidylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 dAMP + sulfite + cytochrome c 80% of the activity with AMP Thiocapsa roseopersicina deoxyadenylyl sulfate + ?
-
 ?
1.8.99.2 deoxyAMP + sulfite + ferricyanide 128% of maximal activity Thiocapsa roseopersicina deoxyadenylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 GMP + sulfite + cytochrome c 9% of the activity with AMP Thiocapsa roseopersicina guanylyl sulfate + ?
-
 ?
1.8.99.2 GMP + sulfite + ferricyanide 51% of the activity with AMP Thiocapsa roseopersicina guanylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 IMP + sulfite + cytochrome c 62% of the activity with AMP Thiocapsa roseopersicina ?
-
 ?
1.8.99.2 IMP + sulfite + ferricyanide 90% of the activity with AMP Thiocapsa roseopersicina ? + ferrocyanide
-
 ?
1.8.99.2 UMP + sulfite + cytochrome c 9% of the activity with AMP Thiocapsa roseopersicina uridylyl sulfate + ?
-
 ?
1.8.99.2 UMP + sulfite + ferricyanide 8% of the activity with AMP Thiocapsa roseopersicina uridylyl sulfate + ferrocyanide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.99.2 8
-
 reaction with ferricyanide Thiocapsa roseopersicina
1.8.99.2 9
-
 reaction with cytochrome c Thiocapsa roseopersicina

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 FAD enzyme contains 1 mol of FAD per mol of enzyme Thiocapsa roseopersicina
1.8.99.2 heme contains 2 heme groups of cytochrome c characterper mol of enzyme Thiocapsa roseopersicina