EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.8.2 | expression in Escherichia coli | Methylophilus methylotrophus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.8.2 | C30A | binds FMN non-covalently | Methylophilus methylotrophus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.8.2 | 0.017 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate, wild-type enzyme | Methylophilus methylotrophus | |
1.5.8.2 | 0.019 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate, recombinant enzyme | Methylophilus methylotrophus | |
1.5.8.2 | 2 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate, mutant C30A | Methylophilus methylotrophus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.5.8.2 | Fe | 4Fe-4S-center | Methylophilus methylotrophus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.8.2 | Methylophilus methylotrophus | - |
- |
- |
1.5.8.2 | Methylophilus methylotrophus W3A1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.8.2 | to homogeneity, chromatography steps | Methylophilus methylotrophus |
1.5.8.2 | to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.8.2 | cell culture | - |
Methylophilus methylotrophus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.8.2 | trimethylamine + H2O + ferricenium hexafluorophosphate | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + ferrocenium hexafluorophosphate | - |
? | |
1.5.8.2 | trimethylamine + H2O + ferricenium hexafluorophosphate | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + ferrocenium hexafluorophosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.8.2 | 17 | - |
trimethylamine | mutant C30A, electron acceptor: ferricenium hexafluorophosphate | Methylophilus methylotrophus | |
1.5.8.2 | 38 | - |
trimethylamine | electron acceptor: ferricenium hexafluorophosphate | Methylophilus methylotrophus | |
1.5.8.2 | 40 | - |
trimethylamine | recombinant enzyme, electron acceptor: ferricenium hexafluorophosphate | Methylophilus methylotrophus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.8.2 | ADP | - |
Methylophilus methylotrophus | |
1.5.8.2 | FMN | covalently bound to Cys30 | Methylophilus methylotrophus |