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Literature summary extracted from

  • Meiberg, J.B.M.; Harder, W.
    Dimethylamine dehydrogenase from Hyphomicrobium X: purification and some properties of a new enzyme that oxidizes secondary amines (1979), J. Gen. Microbiol., 115, 49-58.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.5.8.1 freezing in liquid nitrogen results in a complete loss of activity Hyphomicrobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.1 Dimethylamine 10 mM, 23% inhibition Hyphomicrobium sp.
1.5.8.1 methylamine above 6 mM, slight inhibition Hyphomicrobium sp.
1.5.8.1 phenazine methosulfate serves as electron acceptor, maximal activity at 2.4 mM. Above 2.4 mM inhibition Hyphomicrobium sp.
1.5.8.1 trimethylamine
-
 Hyphomicrobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.1 0.0156
-
 Dimethylamine
-
 Hyphomicrobium sp.
1.5.8.1 0.11
-
 N,N,N,N-tetramethyl-o-phenylenediamine
-
 Hyphomicrobium sp.
1.5.8.1 1.18
-
 phenazine methosulfate
-
 Hyphomicrobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.8.1 91000
-
 2 * 91000, SDS-PAGE Hyphomicrobium sp.
1.5.8.1 176000
-
 gel filtration Hyphomicrobium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.1 Hyphomicrobium sp.
-
 X
-
1.5.8.1 Hyphomicrobium sp. X
-
 X
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.1 partial Hyphomicrobium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.1 culture condition:dimethylamine-grown cell anaerobically Hyphomicrobium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.8.1 0.3638
-
-
 Hyphomicrobium sp.

Storage Stability

EC Number Storage Stability Organism
1.5.8.1 -10°C, 20% v/v ethylene glycol, stable for at least 3 months Hyphomicrobium sp.
1.5.8.1 0°C, 3 months without stabilizer, 50% loss of activity Hyphomicrobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.1 diethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ethylamine + acetaldehyde + reduced acceptor
-
 ?
1.5.8.1 diethylamine + H2O + acceptor at 78.3% of the activity with dimethylamine Hyphomicrobium sp. ethylamine + acetaldehyde + reduced acceptor
-
 ?
1.5.8.1 dimethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. methylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 dimethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. X methylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 dimethylamine + H2O + phenazine methosulfate
-
 Hyphomicrobium sp. methylamine + formaldehyde + reduced phenazine methosulfate
-
 r
1.5.8.1 ethylpropylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ?
-
 ?
1.5.8.1 ethylpropylamine + H2O + acceptor at 6.8% of the activity with dimethylamine Hyphomicrobium sp. ?
-
 ?
1.5.8.1 methylethanolamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ?
-
 ?
1.5.8.1 methylethanolamine + H2O + acceptor at 54.2% of the activity with dimethylamine Hyphomicrobium sp. ?
-
 ?
1.5.8.1 methylethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. ethylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 methylethylamine + H2O + acceptor at 92.1% of the activity with dimethylamine Hyphomicrobium sp. ethylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 methylethylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. X ethylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 methylethylamine + H2O + acceptor at 92.1% of the activity with dimethylamine Hyphomicrobium sp. X ethylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 methylpropylamine + H2O + acceptor acceptor: phenazine methosulfate, phenazine ethosulfate, methylene blue, N,N,N,N-tetramethyl-o-phenylenediamine Hyphomicrobium sp. propylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 methylpropylamine + H2O + acceptor at 12.9% of the activity with dimethylamine Hyphomicrobium sp. propylamine + formaldehyde + reduced acceptor
-
 ?
1.5.8.1 additional information no reaction with cytochrome c Hyphomicrobium sp. ?
-
 ?
1.5.8.1 additional information no reaction with primary and tertiary amines and quarternary ammonium salts Hyphomicrobium sp. ?
-
 ?
1.5.8.1 additional information no reaction with cytochrome c Hyphomicrobium sp. X ?
-
 ?
1.5.8.1 additional information no reaction with primary and tertiary amines and quarternary ammonium salts Hyphomicrobium sp. X ?
-
 ?
1.5.8.1 N,N,N,N-tetramethyl-o-phenylenediamine + H2O + electron-transfer flavoprotein
-
 Hyphomicrobium sp. ?
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.8.1 dimer 2 * 91000, SDS-PAGE Hyphomicrobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.8.1 8.1
-
-
 Hyphomicrobium sp.