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Literature summary extracted from
- Purification and characterization of a cytochrome p450 enzyme from pig liver, catalyzing the phenol oxidative coupling of (R)-reticuline to salutaridine, the critical step in morphine biosynthesis (1995), Heterocycles, 1, 425-440.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.67 | 0.003 | - |
(R)-reticuline | - |
Sus scrofa |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.67 | lysosome | - |
Sus scrofa | 5764 | - |
| 1.14.19.67 | microsome | - |
Mus musculus | - |
- |
| 1.14.19.67 | microsome | - |
Homo sapiens | - |
- |
| 1.14.19.67 | microsome | - |
Rattus norvegicus | - |
- |
| 1.14.19.67 | microsome | - |
Sus scrofa | - |
- |
| 1.14.19.67 | microsome | - |
Bos taurus | - |
- |
| 1.14.19.67 | microsome | - |
Ovis aries | - |
- |
| 1.14.19.67 | mitochondrion | - |
Mus musculus | 5739 | - |
| 1.14.19.67 | mitochondrion | light mitochondrial fraction | Sus scrofa | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.67 | 50000 | - |
x * 50000, SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | Sus scrofa | critical step in morphine biosynthesis | salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.67 | Bos taurus | - |
- |
- |
| 1.14.19.67 | Homo sapiens | - |
very low activity | - |
| 1.14.19.67 | Mus musculus | - |
- |
- |
| 1.14.19.67 | Ovis aries | - |
- |
- |
| 1.14.19.67 | Rattus norvegicus | - |
- |
- |
| 1.14.19.67 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.19.67 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.67 | liver | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | highly regioselective and stereoselective enzyme | Sus scrofa | salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | critical step in morphine biosynthesis | Sus scrofa | salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.67 | (R/S)-orientaline + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sus scrofa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.19.67 | ? | x * 50000, SDS-PAGE | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.67 | cytochrome P450 | - |
Mus musculus | |
| 1.14.19.67 | cytochrome P450 | - |
Homo sapiens | |
| 1.14.19.67 | cytochrome P450 | - |
Rattus norvegicus | |
| 1.14.19.67 | cytochrome P450 | - |
Bos taurus | |
| 1.14.19.67 | cytochrome P450 | - |
Ovis aries | |
| 1.14.19.67 | cytochrome P450 | enzyme contains cytochrome P450 | Sus scrofa | |
| 1.14.19.67 | cytochrome P450 | 20 nmol cytochrome P450 per mg of enzyme | Sus scrofa | |
| 1.14.19.67 | N5-Dinitrophenyl-D-ornithine methyl ester | - |
Bos taurus | |
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