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Literature summary extracted from

  • Steffens, P.; Nagakura, N.; Zenk, M.H.
    Purification and characterization of the berberine bridge enzyme from Berberis beaniana cell cultures (1985), Phytochemistry, 24, 2577-2583.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.3.3 (R)-norreticuline 50% inhibition at 0.02 mM Berberis beaniana
1.21.3.3 (S)-coreximine 50% inhibition at 0.2 mM Berberis beaniana
1.21.3.3 (S)-norreticuline 50% inhibition at 0.001 mM Berberis beaniana
1.21.3.3 (S)-scoulerine
-
 Berberis beaniana
1.21.3.3 Berberine 50% inhibition at 0.004 mM Berberis beaniana
1.21.3.3 diethyldithiocarbamate 50% inhibition at 0.4 mM Berberis beaniana
1.21.3.3 H2O2 50% inhibition at 0.7 M Berberis beaniana
1.21.3.3 Jatrorrhizine 50% inhibition at 0.03 mM Berberis beaniana
1.21.3.3 Na2EDTA 50% inhibition at 6 mM Berberis beaniana
1.21.3.3 o-phenanthroline 50% inhibition at 0.006 mM Berberis beaniana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.3.3 49000
-
 gel filtration Berberis beaniana
1.21.3.3 54000
-
 1 * 54000, SDS-PAGE Berberis beaniana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.3 (S)-reticuline + O2 Embryophyta
-
 (S)-scoulerine + H2O2
-
 ?
1.21.3.3 (S)-reticuline + O2 Berberis beaniana reticuline is the biogenic precursor of the protoberine skeleton (S)-scoulerine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.3 Berberis beaniana
-
-
-
1.21.3.3 Embryophyta
-
 overview of occurence of BBE in Berberidaceae, Ranunculaceae, Menispermaceae, Papaveraceae and Fumariaceae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.3.3
-
 Berberis beaniana

Storage Stability

EC Number Storage Stability Organism
1.21.3.3 -20°C, 320 d, pH 7.4, 50% activity Berberis beaniana
1.21.3.3 25°C, 18 d, pH 7.4, 50% activity Berberis beaniana
1.21.3.3 37°C, 6 d, pH 7.4, 50% activity Berberis beaniana
1.21.3.3 4°C, 150 d, pH 7.4, 50% activity Berberis beaniana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.3 (R,S)-laudanosoline + O2 specific for the (S)-enantiomer Berberis beaniana ? + H2O2
-
 ?
1.21.3.3 (S)-protosinomenine + O2
-
 Berberis beaniana ? + H2O2
-
 ?
1.21.3.3 (S)-reticuline + O2
-
 Embryophyta (S)-scoulerine + H2O2
-
 ?
1.21.3.3 (S)-reticuline + O2 reticuline is the biogenic precursor of the protoberine skeleton Berberis beaniana (S)-scoulerine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.3.3 monomer 1 * 54000, SDS-PAGE Berberis beaniana